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Extracellular secretion of recombinant proteins

Patent ·
OSTI ID:1149687
;
Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.
Research Organization:
National Energy Technology Laboratory (NETL), Pittsburgh, PA, and Morgantown, WV (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
;
Other Award/Contract Number:
AC36-08G028308
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
8,785,159
Application Number:
12/950,442
OSTI ID:
1149687
Country of Publication:
United States
Language:
English

References (13)

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Characterization of multiple promoters and transcript stability in the sacB–sacC gene cluster in Zymomonas mobilis journal May 2009
Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis journal February 2005
Transfer and Expression of an Erwinia chrysanthemi Cellulase Gene in Zymomonas mobilis journal April 1989
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis journal January 1995
Heterologous Expression and Extracellular Secretion of Cellulolytic Enzymes by Zymomonas mobilis journal August 2010
Complete Cellulase System in the Marine Bacterium Saccharophagus degradans Strain 2-40T journal May 2006
Cloning, sequencing, and characterization of the principal acid phosphatase, the phoC+ product, from Zymomonas mobilis. journal February 1989
Use of the tac promoter and lacIq for the controlled expression of Zymomonas mobilis fermentative genes in Escherichia coli and Zymomonas mobilis. journal November 1992
What next for human gene therapy? journal June 2003
The Bacterial Twin-Arginine Translocation Pathway journal October 2006

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