Characterization and Application of a Hybrid Optical Microscopy/Laser Ablation Liquid Vortex Capture/Electrospray Ionization System for Mass Spectrometry Imaging with Sub-micrometer Spatial Resolution

Cahill, John F.; Kertesz, Vilmos; Van Berkel, Gary J.

doi:10.1021/acs.analchem.5b03293

Characterization and Application of a Hybrid Optical Microscopy/Laser Ablation Liquid Vortex Capture/Electrospray Ionization System for Mass Spectrometry Imaging with Sub-micrometer Spatial Resolution

Journal Article · Thu Oct 22 00:00:00 EDT 2015 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.5b03293· OSTI ID:1286876

Cahill, John F. ^[1]; Kertesz, Vilmos ^[1]; Van Berkel, Gary J. ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Herein, a commercial optical microscope, laser microdissection instrument was coupled with an electrospray ionization mass spectrometer via a low profile liquid vortex capture probe to yield a hybrid optical microscopy/mass spectrometry imaging system. The instrument has bright-field and fluorescence microscopy capabilities in addition to a highly focused UV laser beam that is utilized for laser ablation of samples. With this system, material laser ablated from a sample using the microscope was caught by a liquid vortex capture probe and transported in solution for analysis by electrospray ionization mass spectrometry. Both lane scanning and spot sampling mass spectral imaging modes were used. The smallest area the system was able to ablate was ~0.544 μm × ~0.544 μm, achieved by oversampling of the smallest laser ablation spot size that could be obtained (~1.9 μm). With use of a model photoresist surface, known features as small as ~1.5 μm were resolved. The capabilities of the system with real world samples were demonstrated first with a blended polymer thin film containing poly(2-vinylpyridine) and poly(N-vinylcarbazole). Using spot sampling imaging, sub-micrometer sized features (0.62, 0.86, and 0.98 μm) visible by optical microscopy were clearly distinguished in the mass spectral images. A second real world example showed the imaging of trace amounts of cocaine in mouse brain thin tissue sections. Lastly, with use of a lane scanning mode with ~6 μm × ~6 μm data pixels, features in the tissue as small as 15 μm in size could be distinguished in both the mass spectral and optical images.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1286876

Journal Information:: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 21 Vol. 87; ISSN 0003-2700

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

References (37)

Direct imaging of single cells and tissue at sub-cellular spatial resolution using transmission geometry MALDI MS: Single-cell imaging with transmission geometry Zavalin, Andre; Todd, Erik M.; Rawhouser, Patrick D. Journal of Mass Spectrometry, Vol. 47, Issue 11 https://doi.org/10.1002/jms.3108	journal	November 2012
Mass spectrometry imaging under ambient conditions Wu, Chunping; Dill, Allison L.; Eberlin, Livia S. Mass Spectrometry Reviews, Vol. 32, Issue 3 https://doi.org/10.1002/mas.21360	journal	September 2012
Protein and polymer analyses up tom/z 100 000 by laser ionization time-of-flight mass spectrometry Tanaka, Koichi; Waki, Hiroaki; Ido, Yutaka Rapid Communications in Mass Spectrometry, Vol. 2, Issue 8 https://doi.org/10.1002/rcm.1290020802	journal	August 1988
Laser microdissection and atmospheric pressure chemical ionization mass spectrometry coupled for multimodal imaging: LA-APCI/MS imaging Lorenz, Matthias; Ovchinnikova, Olga S.; Kertesz, Vilmos Rapid Communications in Mass Spectrometry, Vol. 27, Issue 13 https://doi.org/10.1002/rcm.6593	journal	May 2013
Transmission geometry laser ablation into a non-contact liquid vortex capture probe for mass spectrometry imaging Ovchinnikova, Olga S.; Bhandari, Deepak; Lorenz, Matthias Rapid Communications in Mass Spectrometry, Vol. 28, Issue 15, p. 1665-1673 https://doi.org/10.1002/rcm.6946	journal	June 2014
Static SIMS for applied surface analysis Brown, A.; Vickerman, J. C. Surface and Interface Analysis, Vol. 6, Issue 1 https://doi.org/10.1002/sia.740060102	journal	February 1984
Improved spatial resolution in the imaging of biological tissue using desorption electrospray ionization Campbell, Dahlia I.; Ferreira, Christina R.; Eberlin, Livia S. Analytical and Bioanalytical Chemistry, Vol. 404, Issue 2 https://doi.org/10.1007/s00216-012-6173-6	journal	June 2012
Peptide structural analysis using continuous Ar cluster and C60 ion beams Aoyagi, Satoka; Fletcher, John S.; Sheraz, Sadia Analytical and Bioanalytical Chemistry, Vol. 405, Issue 21 https://doi.org/10.1007/s00216-013-7139-z	journal	July 2013
Mass spectrometry imaging with high resolution in mass and space Römpp, Andreas; Spengler, Bernhard Histochemistry and Cell Biology, Vol. 139, Issue 6 https://doi.org/10.1007/s00418-013-1097-6	journal	May 2013
High Resolution Tissue Imaging Using the Single-probe Mass Spectrometry under Ambient Conditions Rao, Wei; Pan, Ning; Yang, Zhibo Journal of The American Society for Mass Spectrometry, Vol. 26, Issue 6 https://doi.org/10.1007/s13361-015-1091-4	journal	March 2015
Comparison of Internal Energy Distributions of Ions Created by Electrospray Ionization and Laser Ablation-Liquid Vortex Capture/Electrospray Ionization Cahill, John F.; Kertesz, Vilmos; Ovchinnikova, Olga S. Journal of The American Society for Mass Spectrometry, Vol. 26, Issue 9 https://doi.org/10.1007/s13361-015-1195-x	journal	June 2015
Surface analysis by Secondary Ion Mass Spectrometry (SIMS) Benninghoven, A. Surface Science, Vol. 299-300 https://doi.org/10.1016/0039-6028(94)90658-0	journal	January 1994
Distribution and Clearance of Cocaine in Brain Is Influenced by Genetics Azar, Marc R.; Acar, Nuket; Erwin, V. Gene Pharmacology Biochemistry and Behavior, Vol. 59, Issue 3 https://doi.org/10.1016/S0091-3057(97)00471-1	journal	March 1998
Altair announces new US patent Shepard, Jeff Focus on Catalysts, Vol. 2006, Issue 5, p. 2 https://doi.org/10.1016/S1351-4180(06)71591-4	journal	May 2006
Mass spectrometric imaging of synthetic polymers Crecelius, Anna C.; Vitz, Jürgen; Schubert, Ulrich S. Analytica Chimica Acta, Vol. 808 https://doi.org/10.1016/j.aca.2013.07.033	journal	January 2014
Scaling down the bioimaging of metals by laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS) Becker, J. S.; Niehren, S.; Matusch, A. International Journal of Mass Spectrometry, Vol. 294, Issue 1 https://doi.org/10.1016/j.ijms.2010.03.013	journal	June 2010
AP-MALDI imaging of neuropeptides in mouse pituitary gland with 5μm spatial resolution and high mass accuracy Guenther, Sabine; Römpp, Andreas; Kummer, Wolfgang International Journal of Mass Spectrometry, Vol. 305, Issue 2-3 https://doi.org/10.1016/j.ijms.2010.11.011	journal	August 2011
Imaging of elements and molecules in biological tissues and cells in the low-micrometer and nanometer range Wu, Bei; Becker, J. Sabine International Journal of Mass Spectrometry, Vol. 307, Issue 1-3 https://doi.org/10.1016/j.ijms.2011.01.019	journal	October 2011
Bioimaging of metals in rat brain hippocampus by laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS) using high-efficiency laser ablation chambers Wu, Bei; Becker, J. Sabine International Journal of Mass Spectrometry, Vol. 323-324 https://doi.org/10.1016/j.ijms.2012.06.012	journal	June 2012
MALDI imaging mass spectrometry: Spatial molecular analysis to enable a new age of discovery Gessel, Megan M.; Norris, Jeremy L.; Caprioli, Richard M. Journal of Proteomics, Vol. 107 https://doi.org/10.1016/j.jprot.2014.03.021	journal	July 2014
Application of laser microdissection ICP–MS for high resolution elemental mapping in mouse brain tissue: A comparative study with laser ablation ICP–MS Sussulini, Alessandra; Becker, J. Sabine Talanta, Vol. 132 https://doi.org/10.1016/j.talanta.2014.10.001	journal	January 2015
Ambient desorption ionization mass spectrometry Venter, Andre; Nefliu, Marcela; Graham Cooks, R. TrAC Trends in Analytical Chemistry, Vol. 27, Issue 4 https://doi.org/10.1016/j.trac.2008.01.010	journal	April 2008
Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons Karas, Michael.; Hillenkamp, Franz. Analytical Chemistry, Vol. 60, Issue 20 https://doi.org/10.1021/ac00171a028	journal	October 1988
Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Laskin, Julia; Heath, Brandi S.; Roach, Patrick J. Analytical Chemistry, Vol. 84, Issue 1 https://doi.org/10.1021/ac2021322	journal	December 2011
Single Cell Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Schober, Yvonne; Guenther, Sabine; Spengler, Bernhard Analytical Chemistry, Vol. 84, Issue 15 https://doi.org/10.1021/ac301337h	journal	July 2012
Mechanisms of Real-Time, Proximal Sample Processing during Ambient Ionization Mass Spectrometry Venter, Andre R.; Douglass, Kevin A.; Shelley, Jacob T. Analytical Chemistry, Vol. 86, Issue 1 https://doi.org/10.1021/ac4038569	journal	December 2013
The Single-Probe: A Miniaturized Multifunctional Device for Single Cell Mass Spectrometry Analysis Pan, Ning; Rao, Wei; Kothapalli, Naga Rama Analytical Chemistry, Vol. 86, Issue 19 https://doi.org/10.1021/ac5029038	journal	September 2014
Sample Preparation: A Crucial Factor for the Analytical Performance of Rationally Designed MALDI Matrices Wiangnon, Kanjana; Cramer, Rainer Analytical Chemistry, Vol. 87, Issue 3 https://doi.org/10.1021/ac504412p	journal	January 2015
Mass Spectrometry Imaging of Biomolecular Information Spengler, Bernhard Analytical Chemistry, Vol. 87, Issue 1 https://doi.org/10.1021/ac504543v	journal	December 2014
Nanostructure Initiator Mass Spectrometry: Tissue Imaging and Direct Biofluid Analysis Yanes, Oscar; Woo, Hin-Koon; Northen, Trent R. Analytical Chemistry, Vol. 81, Issue 8 https://doi.org/10.1021/ac802576q	journal	April 2009
Current State and Future Challenges of Mass Spectrometry Imaging for Clinical Research Addie, Ruben D.; Balluff, Benjamin; Bovée, Judith V. M. G. Analytical Chemistry, Vol. 87, Issue 13 https://doi.org/10.1021/acs.analchem.5b00416	journal	April 2015
Co-registered Topographical, Band Excitation Nanomechanical, and Mass Spectral Imaging Using a Combined Atomic Force Microscopy/Mass Spectrometry Platform Ovchinnikova, Olga S.; Tai, Tamin; Bocharova, Vera ACS Nano, Vol. 9, Issue 4 https://doi.org/10.1021/acsnano.5b00659	journal	February 2015
Analysis of Tissue Specimens by Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry in Biological and Clinical Research Norris, Jeremy L.; Caprioli, Richard M. Chemical Reviews, Vol. 113, Issue 4 https://doi.org/10.1021/cr3004295	journal	February 2013
Freeze-Etching and Vapor Matrix Deposition for ToF-SIMS Imaging of Single Cells Piehowski, Paul D.; Kurczy, Michael E.; Willingham, David Langmuir, Vol. 24, Issue 15 https://doi.org/10.1021/la800292e	journal	June 2008
Mass spectrometric imaging of elements in biological tissues by new BrainMet technique—laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS) Wu, Bei; Niehren, Stefan; Becker, J. Sabine Journal of Analytical Atomic Spectrometry, Vol. 26, Issue 8 https://doi.org/10.1039/c1ja10106d	journal	January 2011
Ambient Ionization Mass Spectrometry Huang, Min-Zong; Yuan, Cheng-Hui; Cheng, Sy-Chyi Annual Review of Analytical Chemistry, Vol. 3, Issue 1, p. 43-65 https://doi.org/10.1146/annurev.anchem.111808.073702	journal	June 2010
MALDI TOF imaging mass spectrometry in clinical pathology: A valuable tool for cancer diagnostics (Review) Kriegsmann, JÖRg; Kriegsmann, Mark; Casadonte, Rita International Journal of Oncology, Vol. 46, Issue 3 https://doi.org/10.3892/ijo.2014.2788	journal	December 2014

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Characterization and Application of a Hybrid Optical Microscopy/Laser Ablation Liquid Vortex Capture/Electrospray Ionization System for Mass Spectrometry Imaging with Sub-micrometer Spatial Resolution

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