Continuity of monolayer-bilayer junctions for localization of lipid raft microdomains in model membranes

Ryu, Yong -Sang; Wittenberg, Nathan J.; Suh, Jeng -Hun; Lee, Sang -Wook; Sohn, Youngjoo; Oh, Sang -Hyun; Parikh, Atul N.; Lee, Sin -Doo

doi:10.1038/srep26823

Continuity of monolayer-bilayer junctions for localization of lipid raft microdomains in model membranes

Journal Article · Fri May 27 00:00:00 EDT 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep26823· OSTI ID:1285912

Ryu, Yong -Sang ^[1]; Wittenberg, Nathan J. ^[2]; Suh, Jeng -Hun ^[3]; Lee, Sang -Wook ^[4]; Sohn, Youngjoo ^[5]; Oh, Sang -Hyun ^[6]; Parikh, Atul N. ^[7]; Lee, Sin -Doo ^[3]

Seoul National Univ., Seoul (Korea). School of Electrical Engineering #032; Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Electrical and Computer Engineering; Seoul National Univ., Seoul (Korea). School of Electrical Engineering #032
Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Electrical and Computer Engineering
Seoul National Univ., Seoul (Korea). School of Electrical Engineering #032
Seoul National Univ., Seoul (Korea). School of Electrical Engineering #032; Korea Research Institute of Standards and Science, Daejeon (Republic of Korea). Division of Physical Metrology
Kyung Hee Univ., Seoul (Korea). Inst. of Oriental Medicine, Dept. of Anatomy, College of Korean Medicine
Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Electrical and Computer Engineering, Dept. of Biomedical Engineering
Univ. of California, Davis, CA (United States). Dept. of Biomedical Engineering and Chemical Engineering & Materials Science

We show that the selective localization of cholesterol-rich domains and associated ganglioside receptors prefer to occur in the monolayer across continuous monolayer-bilayer junctions (MBJs) in supported lipid membranes. For the MBJs, glass substrates were patterned with poly(dimethylsiloxane) (PDMS) oligomers by thermally-assisted contact printing, leaving behind 3 nm-thick PDMS patterns. The hydrophobicity of the transferred PDMS patterns was precisely tuned by the stamping temperature. Lipid monolayers were formed on the PDMS patterned surface while lipid bilayers were on the bare glass surface. Due to the continuity of the lipid membranes over the MBJs, essentially free diffusion of lipids was allowed between the monolayer on the PDMS surface and the upper leaflet of the bilayer on the glass substrate. The preferential localization of sphingomyelin, ganglioside GM1 and cholesterol in the monolayer region enabled to develop raft microdomains through coarsening of nanorafts. Furthermore, our methodology provides a simple and effective scheme of non-disruptive manipulation of the chemical landscape associated with lipid phase separations, which leads to more sophisticated applications in biosensors and as cell culture substrates.

View Accepted Manuscript (DOE)

Research Organization:: University of California, Davis

Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-04ER46173

OSTI ID:: 1285912

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 6; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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