Detection of polyaromatic hydrocarbons using DNA intercalation
Conference
·
OSTI ID:127020
- National Institute of Standards and Technology, Gaithersburg, MD (United States)
Polyaromatic hydrocarbons (PAH`S) have be monitored using intercalation of double stranded DNA. Three approaches have been examined. The first, an electrochemical method uses an electroactive intercalating agent. When intercalated into DNA it cannot transfer electrons to an electrode. When displaced by a PAH, it can be detected electrochemically. The second method utilizes fluorescence polarization. A fluorescent intercalating agent, when intercalated into DNA will show increased polarization. When displaced by a competing PAH, a decrease in polarization is observed. The third technique involves evanescent wave technology. Double stranded DNA in close proximity to the wave guide will show a decreased fluorescence when a fluorescent intercalator is displaced by a PAH. Each of these techniques will be described and examples of results presented.
- OSTI ID:
- 127020
- Report Number(s):
- CONF-950402--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of ultraviolet irradiation of DNA on the dissociation transition of the strong DNA-acriflavine complex
Electrothermal vaporization and laser-induced fluorescence for screening of polyaromatic hydrocarbons
Selective strand scission by intercalating drugs at DNA bulges
Journal Article
·
Wed Oct 31 23:00:00 EST 1973
· Photochem. Photobiol., v. 18, no. 5, pp. 437-439
·
OSTI ID:4368469
Electrothermal vaporization and laser-induced fluorescence for screening of polyaromatic hydrocarbons
Journal Article
·
Wed Jul 15 00:00:00 EDT 1987
· Anal. Chem.; (United States)
·
OSTI ID:6182361
Selective strand scission by intercalating drugs at DNA bulges
Journal Article
·
Tue Apr 19 00:00:00 EDT 1988
· Biochemistry; (United States)
·
OSTI ID:7130251