Effect of ultraviolet irradiation of DNA on the dissociation transition of the strong DNA-acriflavine complex

Journal Article · Thu Nov 01 04:00:00 EST 1973 · Photochem. Photobiol., v. 18, no. 5, pp. 437-439

DOI:https://doi.org/10.1111/j.1751-1097.1973.tb06446.x· OSTI ID:4368469

Roth, D

>Fluorescence quenching was used as an expression of intercalative dye binding in an examination of the effects that prior ultraviolet irradiation of DNA has upon the dissociation transition of its complex with acriflavine. The term dissociation transition is applied to the biphasic thermal dissociation kinetics exhibited by DNA complexes with intercalating cations. At room temperatures the dye showed intercalatlve binding to denatured and native DNA. When bound to single-stranded DNA in the incompletely intercalated complex, its thermal dissociation slope was linear over the 25 to 60 deg C range; the free dye levels of fluorescence were restored at 60 deg C. The dissociation curve of the doublestranded complex was biphasic, exhibiting a low rate of increase in fluorescence yield at low temperatures, a transition region at ~60 deg C, and thereafter a rapid increase. These results were taken to indicate that dye fluoresccnce values at 60 deg C could be used to discriminate between single- stranded and double-stranded DNA--acriflavine complexes in the region of A--T pairs. It is suggested that the 27% and 88% increases in fluorescence yield at 60 deg C, which developed with the two experimental uv doses, may be crude expressions of the number of dimers formed between adjacent thymine residues. (UK)

• Research Organization: New York Univ., NY
• NSA Number: NSA-29-018558
• OSTI ID: 4368469
• Journal Information: Photochem. Photobiol., v. 18, no. 5, pp. 437-439, Journal Name: Photochem. Photobiol., v. 18, no. 5, pp. 437-439; ISSN PHCBA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

*DNA-- BIOLOGICAL RADIATION EFFECTS
ACRIFLAVINE
BASES
DIMERS
DISSOCIATION
FLUORESCENCE
N48110* --Life Sciences--Radiation Effects on Biochemicals-- In-Vitro
PHOTON BEAMS
RESPONSE MODIFYING FACTORS
TEMPERATURE DEPENDENCE
THYMINE
ULTRAVIOLET RADIATION