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Title: Transfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethane

Journal Article · · Frontiers in Microbiology
 [1];  [2];  [2];  [3]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Harvard Univ., Cambridge, MA (United States)
  2. Univ. of Strasbourg (France)
  3. Harvard Univ., Cambridge, MA (United States); Univ. of Idaho, Moscow, ID (United States)
+ Show Author Affiliations

Chloromethane is an ozone-depleting gas, produced predominantly from natural sources, that provides an important environmental niche for microbes capable of consuming it. Chloromethane catabolism has been difficult to study owing to the challenging genetics of its native microbial hosts. Since the pathways for chloromethane catabolism show evidence of horizontal gene transfer, we reproduced this transfer process in the laboratory to generate new chloromethane-catabolizing strains in tractable hosts. Here, we demonstrate that six putative accessory genes improve chloromethane catabolism, though heterologous expression of only one of the six is strictly necessary for growth on chloromethane. In contrast to growth of Methylobacterium strains with the closely-related compound dichloromethane, we find that chloride export does not limit growth on chloromethane and, in general, that the ability of a strain to grow on dichloromethane is uncorrelated with its ability to grow on chloromethane. Finally, this heterologous expression system allows us to investigate the components required for effective chloromethane catabolism and the factors that limit effective catabolism after horizontal transfer.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC05-00OR22725; F32 GM106629
OSTI ID:
1265893
Journal Information:
Frontiers in Microbiology, Vol. 7; ISSN 1664-302X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

References (39)

Chloromethane Utilization Gene Cluster fromHyphomicrobium chloromethanicum Strain CM2T and Development of Functional Gene Probes To Detect Halomethane-Degrading Bacteria journal January 2001
Development of an insertional expression vector system for Methylobacterium extorquens AM1 and generation of null mutants lacking mtdA and/or fch journal January 2004
The 380 kb pCMU01 Plasmid Encodes Chloromethane Utilization Genes and Redundant Genes for Vitamin B12- and Tetrahydrofolate-Dependent Chloromethane Metabolism in Methylobacterium extorquens CM4: A Proteomic and Bioinformatics Study journal April 2013
New insight into the atmospheric chloromethane budget gained using stable carbon isotope ratios journal January 2005
Competitiveness of Diverse Methylobacterium Strains in the Phyllosphere of Arabidopsis thaliana and Identification of Representative Models, Including M. extorquens PA1 journal August 2010
Phylogeny Poorly Predicts the Utility of a Challenging Horizontally Transferred Gene in Methylobacterium Strains journal March 2014
Development of an Optimized Medium, Strain and High-Throughput Culturing Methods for Methylobacterium extorquens journal April 2013
Purification of the Formate-Tetrahydrofolate Ligasefrom Methylobacterium extorquens AM1 and Demonstrationof Its Requirement for MethylotrophicGrowth journal December 2003
Complete Genome Sequence of the Chloromethane-Degrading Hyphomicrobium sp. Strain MC1 journal August 2011
Probing the diversity of chloromethane-degrading bacteria by comparative genomics and isotopic fractionation journal October 2014
Mapping the Fitness Landscape of Gene Expression Uncovers the Cause of Antagonism and Sign Epistasis between Adaptive Mutations journal February 2014
Complete Genome Sequences of Six Strains of the Genus Methylobacterium journal August 2012
Identification of Methyl Halide-Utilizing Genes in the Methyl Bromide-Utilizing Bacterial Strain IMB-1 Suggests a High Degree of Conservation of Methyl Halide-Specific Genes in Gram-Negative Bacteria journal April 2001
Methyl Chloride: Naturally Occurring Toxicant and C-1 Growth Substrate journal April 1986
Microbial growth on C1 compounds. 1. Isolation and characterization of Pseudomonas AM 1 journal December 1961
Analysis of genes involved in methyl halide degradation in Aminobacter lissarensis CC495 journal October 2005
Chloromethane Metabolism by Methylobacterium sp. Strain CM4 journal May 1998
Molecular cloning of a malyl coenzyme A lyase gene from Pseudomonas sp. strain AM1, a facultative methylotroph. journal January 1984
Isolation and initial characterization of aerobic chloromethane-utilizing bacteria journal September 1996
Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria journal August 2001
Methylamine Utilization via the N-Methylglutamate Pathway in Methylobacterium extorquens PA1 Involves a Novel Flow of Carbon through C1 Assimilation and Dissimilation Pathways journal September 2014
Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides×nigra DN34) journal July 2004
A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane journal April 1999
Specialized bacterial strains for the removal of dichloromethane from industrial waste journal January 1985
Sequence analysis and expression of the bacterial dichloromethane dehalogenase structural gene, a member of the glutathione S-transferase supergene family. journal January 1990
Formate as the Main Branch Point for Methylotrophic Metabolism in Methylobacterium extorquens AM1 journal May 2008
C1 Transfer Enzymes and Coenzymes Linking Methylotrophic Bacteria and Methanogenic Archaea journal July 1998
Chloromethane-Induced Genes Define a Third C1 Utilization Pathway in Methylobacterium chloromethanicum CM4 journal July 2002
Dichloromethane metabolism and C1 utilization genes in Methylobacterium strains The GenBank accession numbers for the sequences determined in this work are AJ421476 and AJ421477. journal June 2002
A strong source of methyl chloride to the atmosphere from tropical coastal land journal January 2000
Mapping the Fitness Landscape of Gene Expression Uncovers the Cause of Antagonism and Sign Epistasis between Adaptive Mutations text January 2014
ASYMMETRIC, BIMODAL TRADE-OFFS DURING ADAPTATION OF METHYLOBACTERIUM TO DISTINCT GROWTH SUBSTRATES journal November 2009
Flux Analysis Uncovers Key Role of Functional Redundancy in Formaldehyde Metabolism journal January 2005
Competitiveness of Diverse Methylobacterium Strains in the Phyllosphere of Arabidopsis thaliana and Identification of Representative Models, Including M. extorquens PA1 text January 2010
Evidence for the presence of a CmuA methyltransferase pathway in novel marine methyl halide-oxidizing bacteria: Novel marine methyl halide-oxidizing bacteria journal March 2005
Detection and isolation of chloromethane-degrading bacteria from the Arabidopsis thaliana phyllosphere, and characterization of chloromethane utilization genes: Chloromethane-degrading bacteria from A. thaliana journal June 2011
Elucidation of the Role of the Methylene-Tetrahydromethanopterin Dehydrogenase MtdA in the Tetrahydromethanopterin-Dependent Oxidation Pathway in Methylobacterium extorquens AM1 journal March 2013
Chloromethane:tetrahydrofolate methyl transfer by two proteins from Methylobacterium chloromethanicum strain CM4: Chloromethane dehalogenase journal May 2001
Patterns of cell division, DNA base compositions, and fine structures of some radiation-resistant vegetative bacteria found in food journal January 1979

Cited By (4)

Methanol consumption drives the bacterial chloromethane sink in a forest soil journal July 2018
Diversity and composition of cave methanotrophic communities journal June 2022
Microbial phenotypic heterogeneity in response to a metabolic toxin: Continuous, dynamically shifting distribution of formaldehyde tolerance in Methylobacterium extorquens populations journal November 2019
Microbial phenotypic heterogeneity in response to a metabolic toxin: continuous, dynamically shifting distribution of formaldehyde tolerance in Methylobacterium extorquens populations journal September 2019

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