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Title: Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park

Abstract

Geobacillus thermoglucosidasius Y4.1MC1 was isolated from a boiling spring in the lower geyser basin of Yellowstone National Park. We present this species is of interest because of its metabolic versatility. The genome consists of one circular chromosome of 3,840,330 bp and a circular plasmid of 71,617 bp with an average GC content of 44.01%. The genome is available in the GenBank database (NC_014650.1 and NC_014651.1). In addition to the expected metabolic pathways for sugars and amino acids, the Y4.1MC1 genome codes for two separate carbon monoxide utilization pathways, an aerobic oxidation pathway and an anaerobic reductive acetyl CoA (Wood-Ljungdahl) pathway. This is the first report of a nonanaerobic organism with the Wood-Ljungdahl pathway. Also, this anaerobic pathway permits the strain to utilize H 2 and fix CO 2 present in the hot spring environment. Y4.1MC1 and its related species may play a significant role in carbon capture and sequestration in thermophilic ecosystems and may open up new routes to produce biofuels and chemicals from CO, H 2, and CO 2.

Authors:
 [1];  [2];  [2];  [3];  [3];  [4]
  1. C5.6 Technologies Inc., Middleton, WI (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division.
  4. Lucigen Corporation, Middleton, WI (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; Work for Others (WFO)
OSTI Identifier:
1265371
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
BioEnergy Research
Additional Journal Information:
Journal Volume: 8; Journal Issue: 3; Journal ID: ISSN 1939-1234
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; Carbon monoxide . Carbon fixation . Wood-Ljungdahl pathway . YellowstoneNational Park . Geobacillus thermoglucosidasius

Citation Formats

Brumm, Phillip, Land, Miriam L., Hauser, Loren John, Jeffries, Cynthia D., Chang, Yun-Juan, and Mead, David A.. Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park. United States: N. p., 2015. Web. doi:10.1007/s12155-015-9585-2.
Brumm, Phillip, Land, Miriam L., Hauser, Loren John, Jeffries, Cynthia D., Chang, Yun-Juan, & Mead, David A.. Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park. United States. doi:10.1007/s12155-015-9585-2.
Brumm, Phillip, Land, Miriam L., Hauser, Loren John, Jeffries, Cynthia D., Chang, Yun-Juan, and Mead, David A.. Tue . "Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park". United States. doi:10.1007/s12155-015-9585-2. https://www.osti.gov/servlets/purl/1265371.
@article{osti_1265371,
title = {Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park},
author = {Brumm, Phillip and Land, Miriam L. and Hauser, Loren John and Jeffries, Cynthia D. and Chang, Yun-Juan and Mead, David A.},
abstractNote = {Geobacillus thermoglucosidasius Y4.1MC1 was isolated from a boiling spring in the lower geyser basin of Yellowstone National Park. We present this species is of interest because of its metabolic versatility. The genome consists of one circular chromosome of 3,840,330 bp and a circular plasmid of 71,617 bp with an average GC content of 44.01%. The genome is available in the GenBank database (NC_014650.1 and NC_014651.1). In addition to the expected metabolic pathways for sugars and amino acids, the Y4.1MC1 genome codes for two separate carbon monoxide utilization pathways, an aerobic oxidation pathway and an anaerobic reductive acetyl CoA (Wood-Ljungdahl) pathway. This is the first report of a nonanaerobic organism with the Wood-Ljungdahl pathway. Also, this anaerobic pathway permits the strain to utilize H2 and fix CO2 present in the hot spring environment. Y4.1MC1 and its related species may play a significant role in carbon capture and sequestration in thermophilic ecosystems and may open up new routes to produce biofuels and chemicals from CO, H2, and CO2.},
doi = {10.1007/s12155-015-9585-2},
journal = {BioEnergy Research},
number = 3,
volume = 8,
place = {United States},
year = {Tue Feb 10 00:00:00 EST 2015},
month = {Tue Feb 10 00:00:00 EST 2015}
}

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Cited by: 4 works
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Works referenced in this record:

Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation
journal, December 2008

  • Ragsdale, Stephen W.; Pierce, Elizabeth
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 12, p. 1873-1898
  • DOI: 10.1016/j.bbapap.2008.08.012

From The Cover: Hydrogen and bioenergetics in the Yellowstone geothermal ecosystem
journal, January 2005

  • Spear, J. R.; Walker, J. J.; McCollom, T. M.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 7, p. 2555-2560
  • DOI: 10.1073/pnas.0409574102

MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004

  • Edgar, R. C.
  • Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
  • DOI: 10.1093/nar/gkh340

The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)
journal, July 2008