Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park

Brumm, Phillip; Land, Miriam L.; Hauser, Loren J.; Jeffries, Cynthia D.; Chang, Yun-Juan; Mead, David A.

doi:10.1186/s40793-015-0075-0

Title: Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park

Journal Article · Mon Oct 19 00:00:00 EDT 2015 · Standards in Genomic Sciences

DOI:https://doi.org/10.1186/s40793-015-0075-0· OSTI ID:1259497

Brumm, Phillip ^[1]; Land, Miriam L. ^[2]; Hauser, Loren J. ^[2]; Jeffries, Cynthia D. ^[3]; Chang, Yun-Juan ^[3]; Mead, David A. ^[4]

C5.6 Technologies Inc., Middleton, WI (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Lucigen Corporation, Middleton, WI (United States)

We isolated geobacillus sp. Y412MC52 from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). Based on 16S rRNA genes and average nucleotide identity, Geobacillus sp. Y412MC52 and the related Geobacillus sp. Y412MC61 appear to be members of a new species of Geobacillus. Moreover, te genome of Geobacillus sp. Y412MC52 consists of one circular chromosome of 3,628,883 bp, an average G + C content of 52 % and one circular plasmid of 45,057 bp and an average G + C content of 45 %. Y412MC52 possesses arabinan, arabinoglucuronoxylan, and aromatic acid degradation clusters for degradation of hemicellulose from biomass. Finally, we present transport and utilization clusters for other carbohydrates including starch, cellobiose, and - and -galactooligosaccharides.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FC02-07ER64494; AC02-05CH11231; AC52-07NA27344; AC02-06NA25396; AC05-00OR22725

OSTI ID:: 1259497

Alternate ID(s):: OSTI ID: 1265845

Journal Information:: Standards in Genomic Sciences, Vol. 10, Issue 1; ISSN 1944-3277

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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Title: Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park

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