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Title: Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park

Abstract

We isolated geobacillus sp. Y412MC52 from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). Based on 16S rRNA genes and average nucleotide identity, Geobacillus sp. Y412MC52 and the related Geobacillus sp. Y412MC61 appear to be members of a new species of Geobacillus. Moreover, te genome of Geobacillus sp. Y412MC52 consists of one circular chromosome of 3,628,883 bp, an average G + C content of 52 % and one circular plasmid of 45,057 bp and an average G + C content of 45 %. Y412MC52 possesses arabinan, arabinoglucuronoxylan, and aromatic acid degradation clusters for degradation of hemicellulose from biomass. Finally, we present transport and utilization clusters for other carbohydrates including starch, cellobiose, and - and -galactooligosaccharides.

Authors:
 [1];  [2];  [2];  [3];  [3];  [4]
  1. C5.6 Technologies Inc., Middleton, WI (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  4. Lucigen Corporation, Middleton, WI (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1259497
Alternate Identifier(s):
OSTI ID: 1265845
Grant/Contract Number:  
FC02-07ER64494; AC02-05CH11231; AC52-07NA27344; AC02-06NA25396; AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Standards in Genomic Sciences
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1944-3277
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Geobacillus sp. Y412MC52; Obsidian hot spring; Biomass; Arabinan; Xylan; G. thermocatenulatus

Citation Formats

Brumm, Phillip, Land, Miriam L., Hauser, Loren J., Jeffries, Cynthia D., Chang, Yun-Juan, and Mead, David A. Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park. United States: N. p., 2015. Web. doi:10.1186/s40793-015-0075-0.
Brumm, Phillip, Land, Miriam L., Hauser, Loren J., Jeffries, Cynthia D., Chang, Yun-Juan, & Mead, David A. Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park. United States. doi:10.1186/s40793-015-0075-0.
Brumm, Phillip, Land, Miriam L., Hauser, Loren J., Jeffries, Cynthia D., Chang, Yun-Juan, and Mead, David A. Mon . "Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park". United States. doi:10.1186/s40793-015-0075-0. https://www.osti.gov/servlets/purl/1259497.
@article{osti_1259497,
title = {Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park},
author = {Brumm, Phillip and Land, Miriam L. and Hauser, Loren J. and Jeffries, Cynthia D. and Chang, Yun-Juan and Mead, David A.},
abstractNote = {We isolated geobacillus sp. Y412MC52 from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). Based on 16S rRNA genes and average nucleotide identity, Geobacillus sp. Y412MC52 and the related Geobacillus sp. Y412MC61 appear to be members of a new species of Geobacillus. Moreover, te genome of Geobacillus sp. Y412MC52 consists of one circular chromosome of 3,628,883 bp, an average G + C content of 52 % and one circular plasmid of 45,057 bp and an average G + C content of 45 %. Y412MC52 possesses arabinan, arabinoglucuronoxylan, and aromatic acid degradation clusters for degradation of hemicellulose from biomass. Finally, we present transport and utilization clusters for other carbohydrates including starch, cellobiose, and - and -galactooligosaccharides.},
doi = {10.1186/s40793-015-0075-0},
journal = {Standards in Genomic Sciences},
issn = {1944-3277},
number = 1,
volume = 10,
place = {United States},
year = {2015},
month = {10}
}

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    Works referencing / citing this record:

    Peculiarities and biotechnological potential of environmental adaptation by Geobacillus species
    journal, October 2018