Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Journal Article · · Nature (London)
DOI:https://doi.org/10.1038/nature14656· OSTI ID:1260958
 [1];  [1];  [1];  [1];  [2];  [3];  [4];  [4];  [4];  [3];  [5];  [1];  [1];  [6];  [1];  [7];  [8];  [8];  [9];  [10] more »;  [11];  [11];  [1];  [1];  [1];  [1];  [1];  [12];  [12];  [13];  [4];  [14];  [14];  [14];  [14];  [2];  [2];  [2];  [15];  [2];  [16];  [2];  [2];  [17];  [17];  [17];  [18];  [19];  [20];  [21];  [21];  [22];  [23];  [24];  [25];  [7];  [7];  [21];  [17];  [26];  [14];  [2];  [14];  [27];  [20];  [11];  [8];  [9];  [28];  [3];  [1];  [29] « less
  1. Van Andel Research Inst. (VARI), Grand Rapids, MI (United States)
  2. Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Dept. of Chemistry and Biochemistry
  3. Univ. of Southern California, Los Angeles, CA (United States). Bridge Inst., Dept. of Chemistry
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL), Joint Center for Structural Genomics
  6. Van Andel Research Inst. (VARI), Grand Rapids, MI (United States); National Univ. of Singapore (Singapore). Yong Loo Lin School of Medicine, Dept. of Obstetrics & Gynecology
  7. New York Structural Biology Center (NYSBC), New York, NY (United States)
  8. Scripps Research Inst., Jupiter, FL (United States). Dept. of Molecular Therapeutics
  9. Univ. of California, Los Angeles, CA (United States). Jules Stein Eye Inst.
  10. Univ. of Toronto, ON (Canada). Dept. of Biochemistry
  11. Vanderbilt Univ., Nashville, TN (United States). Dept. of Pharmacology
  12. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  13. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS); NSF Science and Technology Center, Buffalo, NY (United States). BioXFEL
  14. Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Dept. of Chemistry and Biochemistry; Arizona State Univ., Tempe, AZ (United States). Dept. of Physics
  15. Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Dept. of Chemistry and Biochemistry; Beijing Computational Science Research Center, Beijing (China)
  16. Arizona State Univ., Tempe, AZ (United States). Biodesign Inst., Dept. of Chemistry and Biochemistry; Univ. of Wisconsin-Milwaukee, Milwaukee, WI (United States). Dept. of Physics
  17. Chinese Academy of Sciences, Shanghai (China). Shanghai Inst. of Materia Medica, State Key Lab. of Drug Research
  18. National Univ. of Singapore (Singapore). Yong Loo Lin School of Medicine, Dept. of Obstetrics & Gynecology
  19. Paul Scherrer Inst. (PSI), Villigen (Switzerland). Swiss Light Source
  20. Univ. of Southern California, Los Angeles, CA (United States). Bridge Inst., Dept. of Biological Sciences
  21. Trinity College, Dublin, (Ireland). School of Medicine and School of Biochemistry and Immunology
  22. NSF Science and Technology Center, Buffalo, NY (United States). BioXFEL; Univ. of Chicago, IL (United States). Ben May Dept. for Cancer Research
  23. Paul Scherrer Inst., Villigen (Switzerland). Lab. of Biomolecular Research
  24. Univ. of Konstanz, Konstanz (Germany). Dept. of Biology
  25. Chinese Academy of Sciences (CAS), Beijing (China). Inst. of High Energy Physics, Beijing Synchrotron Radiation Facility
  26. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Centre for Ultrafast Imaging, Hamburg (Germany)
  27. Univ. of Toronto, ON (Canada). Dept. of Biochemistry; Univ. of Toronto, ON (Canada). Dept. of Molecular Genetics
  28. Univ. of Southern California, Los Angeles, CA (United States). Bridge Inst., Dept. of Chemistry; Univ. of Southern California, Los Angeles, CA (United States). Bridge Inst., Dept. of Biological Sciences; ShanghaiTech Univ., Shanghai (China)
  29. Van Andel Research Inst. (VARI), Grand Rapids, MI (United States); Chinese Academy of Sciences (CAS), Shanghai (China). Shanghai Inst. of Materia Medica
+ Show Author Affiliations
G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin-arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a ~20° rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. In conclusion, this structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology.
Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Inst. of Health (NIH)
Grant/Contract Number:
AC02-76SF00515; AC02-06CH11357
OSTI ID:
1260958
Alternate ID(s):
OSTI ID: 1259890
Journal Information:
Nature (London), Journal Name: Nature (London) Journal Issue: 7562 Vol. 523; ISSN 0028-0836
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

References (116)

Structural origin of weakly ordered nitroxide motion in spin-labeled proteins journal May 2009
Time Window Expansion for HDX Analysis of an Intrinsically Disordered Protein journal July 2013
Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography journal May 2015
Crystal Structure of a Thermally Stable Rhodopsin Mutant journal October 2007
Rational design of lipid for membrane protein crystallization journal November 2004
Automated molecular microscopy: The new Leginon system journal July 2005
Appion: An integrated, database-driven pipeline to facilitate EM image processing journal April 2009
The phase diagram of the monoolein/water system: metastability and equilibrium aspects journal February 2000
Ulp1-SUMO Crystal Structure and Genetic Analysis Reveal Conserved Interactions and a Regulatory Element Essential for Cell Growth in Yeast journal May 2000
Constitutive Activation of Opsin by Mutation of Methionine 257 on Transmembrane Helix 6 † journal June 1998
Single-Cysteine Substitution Mutants at Amino Acid Positions 55−75, the Sequence Connecting the Cytoplasmic Ends of Helices I and II in Rhodopsin:  Reactivity of the Sulfhydryl Groups and Their Derivatives Identifies a Tertiary Structure that Changes upon Light-Activation † , ‡ journal June 1999
Structure and activation of rhodopsin journal January 2012
Crystal structure of the ligand-free G-protein-coupled receptor opsin journal June 2008
Crystal structure of metarhodopsin II journal March 2011
The structural basis of agonist-induced activation in constitutively active rhodopsin journal March 2011
Crystal structure of the β2 adrenergic receptor–Gs protein complex journal July 2011
Visualization of arrestin recruitment by a G-protein-coupled receptor journal June 2014
Distinct loops in arrestin differentially regulate ligand binding within the GPCR opsin journal January 2012
Image processing for electron microscopy single-particle analysis using XMIPP journal May 2008
Crystallizing membrane proteins using lipidic mesophases journal April 2009
Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity journal August 2022
Artificial signaling in mammalian cells enabled by prokaryotic two-component system journal December 2019
Structure and function of the visual arrestin oligomer journal March 2007
Chromosomal transposition of PiggyBac in mouse embryonic stem cells journal June 2008
High-resolution distance mapping in rhodopsin reveals the pattern of helix movement due to activation journal May 2008
Involvement of distinct arrestin-1 elements in binding to different functional forms of rhodopsin journal December 2012
Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line journal October 2002
Phosphodiesterase activation by photoexcited rhodopsin is quenched when rhodopsin is phosphorylated and binds the intrinsic 48-kDa protein of rod outer segments. journal March 1986
Potent Peptide Analogues of a G Protein Receptor-binding Region Obtained with a Combinatorial Library journal January 1996
Monomeric Rhodopsin Is Sufficient for Normal Rhodopsin Kinase (GRK1) Phosphorylation and Arrestin-1 Binding journal October 2010
Indexing in single-crystal diffractometry with an obstinate list of reflections journal April 1992
Features and development of Coot journal March 2010
REFMAC 5 for the refinement of macromolecular crystal structures journal March 2011
Crystallographic data processing for free-electron laser sources journal June 2013
Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data journal May 2014
Biased signalling and allosteric machines: new vistas and challenges for drug discovery: 7TMRs as allosteric proteins journal February 2012
High-Resolution Crystal Structure of an Engineered Human  2-Adrenergic G Protein-Coupled Receptor journal October 2007
Structure of an Agonist-Bound Human A2A Adenosine Receptor journal March 2011
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography journal May 2012
Organization of the Influenza Virus Replication Machinery journal November 2012
Structural Basis for Molecular Recognition at Serotonin Receptors journal March 2013
Structure-Function of the G Protein–Coupled Receptor Superfamily journal January 2013
Ultra-precise characterization of LCLS hard X-ray focusing mirrors by high resolution slope measuring deflectometry journal January 2012
Data from: Endocranial anatomy of a new fossil porpoise (Odontoceti: Phocoenidae) from the Pliocene San Diego Formation of California dataset January 2014
X-ray computed tomography datasets for forensic analysis of vertebrate fossils collection January 2016
Topographic study of arrestin using differential chemical modifications and hydrogen/deuterium exchange journal December 1994
Development of a new physics-based internal coordinate mechanics force field and its application to protein loop modeling journal November 2010
G-protein-coupled Receptor Kinases book January 2004
G Protein-Coupled Receptor Kinases book January 2016
HDX Workbench: Software for the Analysis of H/D Exchange MS Data journal June 2012
A simple mechanical mixer for small viscous lipid-containing samples journal September 1998
Visual arrestin interaction with rhodopsin. Sequential multisite binding ensures strict selectivity toward light-activated phosphorylated rhodopsin journal June 1993
A Model for Arrestin’s Regulation: The 2.8 Å Crystal Structure of Visual Arrestin journal April 1999
The phase diagram of the monoolein/water system: metastability and equilibrium aspects journal February 2000
[19] Integrated methods for the construction of three-dimensional models and computational probing of structure-function relations in G protein-coupled receptors book January 1995
Ulp1-SUMO Crystal Structure and Genetic Analysis Reveal Conserved Interactions and a Regulatory Element Essential for Cell Growth in Yeast journal May 2000
Rapid and Facile Recombinant Expression of Bovine Rhodopsin in HEK293S GnTI− Cells Using a PiggyBac Inducible System book January 2015
Genetically Encoded Chemical Probes in Cells Reveal the Binding Path of Urocortin-I to CRF Class B GPCR journal December 2013
Structural Impact of the E113Q Counterion Mutation on the Activation and Deactivation Pathways of the G Protein-coupled Receptor Rhodopsin journal June 2008
DoG Picker and TiltPicker: Software tools to facilitate particle selection in single particle electron microscopy journal May 2009
The functional cycle of visual arrestins in photoreceptor cells journal November 2011
Membrane Protein Crystallization in Lipidic Mesophases with Tailored Bilayers journal December 2004
Microscale Fluorescent Thermal Stability Assay for Membrane Proteins journal March 2008
Protein Conformation Ensembles Monitored by HDX Reveal a Structural Rationale for Abscisic Acid Signaling Protein Affinities and Activities journal February 2013
An Opsin Mutant with Increased Thermal Stability † journal February 2003
Motion of Spin Label Side Chains in Cellular Retinol-Binding Protein:  Correlation with Structure and Nearest-Neighbor Interactions in an Antiparallel β-Sheet † journal March 2004
Development of an Automated High Throughput LCP-FRAP Assay to Guide Membrane Protein Crystallization in Lipid Mesophases journal April 2011
X-ray crystal structure of arrestin from bovine rod outer segments journal February 1998
Rhodopsin dimers in native disc membranes journal January 2003
Crystal structure of opsin in its G-protein-interacting conformation journal September 2008
Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide journal April 2013
Crystal structure of pre-activated arrestin p44 journal April 2013
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography journal February 2014
Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography journal August 2014
PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome journal December 2019
Insights into the activation mechanism of the visual receptor rhodopsin journal March 2012
G Protein-Coupled Receptor Kinases journal August 1994
Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin journal March 2006
Each rhodopsin molecule binds its own arrestin journal February 2007
The genetic design of signaling cascades to record receptor activation journal December 2007
Conformational dynamics of helix 8 in the GPCR rhodopsin controls arrestin activation in the desensitization process journal October 2011
Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II journal December 2011
Conformation of receptor-bound visual arrestin journal October 2012
Simple piggyBac transposon-based mammalian cell expression system for inducible protein production journal March 2013
Functional map of arrestin-1 at single amino acid resolution journal January 2014
Monomeric Rhodopsin Is Sufficient for Normal Rhodopsin Kinase (GRK1) Phosphorylation and Arrestin-1 Binding journal October 2010
Transducin Activation by Nanoscale Lipid Bilayers Containing One and Two Rhodopsins journal March 2007
The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) journal March 2010
Phaser crystallographic software journal July 2007
CrystFEL : a software suite for snapshot serial crystallography journal March 2012
Indexing in single-crystal diffractometry with an obstinate list of reflections journal April 1992
Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals journal February 2011
A statistic for local intensity differences: robustness to anisotropy and pseudo-centering and utility for detecting twinning journal June 2003
MolProbity : all-atom structure validation for macromolecular crystallography journal December 2009
Integration, scaling, space-group assignment and post-refinement journal January 2010
PHENIX: a comprehensive Python-based system for macromolecular structure solution journal January 2010
Features and development of Coot journal March 2010
iMOSFLM : a new graphical interface for diffraction-image processing with MOSFLM journal March 2011
REFMAC 5 for the refinement of macromolecular crystal structures journal March 2011
Statistical quality indicators for electron-density maps journal March 2012
Crystallographic data processing for free-electron laser sources journal June 2013
Space-group and origin ambiguity in macromolecular structures with pseudo-symmetry and its treatment with the program Zanuda journal August 2014
Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data journal May 2014
A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation journal January 1999
Biased Signaling Pathways in  2-Adrenergic Receptor Characterized by 19F-NMR journal January 2012
Linking Crystallographic Model and Data Quality journal May 2012
Structural Features for Functional Selectivity at Serotonin Receptors journal March 2013
Serial Femtosecond Crystallography of G Protein-Coupled Receptors journal December 2013
Crystal Structure of Rhodopsin: A G Protein-Coupled Receptor journal August 2000
Biased Signaling Pathways in β2-Adrenergic Receptor Characterized by 19F-NMR book August 2021
G Protein–Coupled Receptor Kinases journal June 1998
Molecular Mechanism of β-Arrestin-Biased Agonism at Seven-Transmembrane Receptors journal February 2012
Ultra-precise characterization of LCLS hard X-ray focusing mirrors by high resolution slope measuring deflectometry journal January 2012
Structural Basis for Molecular Recognition at Serotonin Receptors text January 2013
Structural Features for Functional Selectivity at Serotonin Receptors text January 2013
PHENIX: a comprehensive Python-based system for macromolecular structure solution. text January 2010

Cited By (169)

Arrestins: structural disorder creates rich functionality journal February 2018
Pharmacogenomics of GPCR Drug Targets journal January 2018
Structural Basis for Allosteric Ligand Recognition in the Human CC Chemokine Receptor 7 journal August 2019
Drug Binding Poses Relate Structure with Efficacy in the μ Opioid Receptor journal June 2017
The structural basis of the dominant negative phenotype of the Gαi1β1γ2 G203A/A326S heterotrimer journal August 2016
Structural basis for selectivity and diversity in angiotensin II receptors journal April 2017
Evolutionary action and structural basis of the allosteric switch controlling β2AR functional selectivity journal December 2017
Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR journal January 2018
Lack of beta-arrestin signaling in the absence of active G proteins journal January 2018
Distinct G protein-coupled receptor phosphorylation motifs modulate arrestin affinity and activation and global conformation journal March 2019
Hologenomic adaptations underlying the evolution of sanguivory in the common vampire bat journal February 2018
New views on phototransduction from atomic force microscopy and single molecule force spectroscopy on native rods journal September 2017
Rethinking the Origin of Primates by Reconstructing Their Diel Activity Patterns Using Genetics and Morphology journal September 2017
The orientation and stability of the GPCR-Arrestin complex in a lipid bilayer journal December 2017
A host dTMP-bound structure of T4 phage dCMP hydroxymethylase mutant using an X-ray free electron laser journal November 2019
Viscosity-adjustable grease matrices for serial nanocrystallography journal January 2020
X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex journal April 2016
Structure and dynamics of a constitutively active neurotensin receptor journal December 2016
Dimerization of the transmembrane domain of amyloid precursor protein is determined by residues around the γ-secretase cleavage sites journal September 2017
State-Dependent Lipid Interactions with the A2a Receptor Revealed by MD Simulations Using In Vivo -Mimetic Membranes posted_content July 2018
Seeing β-arrestin in action: The role of β-arrestins in Histamine 1 Receptor signaling journal September 2019
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography journal August 2016
Native phasing of x-ray free-electron laser data for a G protein–coupled receptor journal September 2016
Molecular Defects of the Disease-Causing Human Arrestin-1 C147F Mutant journal January 2018
Improving virtual screening of G protein-coupled receptors via ligand-directed modeling journal November 2017
Receptor, Ligand and Transducer Contributions to Dopamine D2 Receptor Functional Selectivity journal October 2015
GPCR-SAS: A web application for statistical analyses on G protein-coupled receptors sequences journal July 2018
Melanopsin Carboxy-terminus phosphorylation plasticity and bulk negative charge, not strict site specificity, achieves phototransduction deactivation journal April 2020
Structural Studies of G Protein-Coupled Receptors journal October 2015
Endocrinology and the brain: corticotropin-releasing hormone signaling journal August 2017
Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound β1-adrenergic receptor. journalarticle January 2017
Evaluation of serial crystallographic structure determination within megahertz pulse trains text January 2019
Enhanced Mutant Compensates for Defects in Rhodopsin Phosphorylation in the Presence of Endogenous Arrestin-1 journal June 2018
Molecular Mechanisms of GPCR Signaling: A Structural Perspective journal November 2017
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography journal September 2016
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons text January 2017
Recent Progress in Understanding the Conformational Mechanism of Heterotrimeric G Protein Activation journal January 2017
Arrestin-mediated signaling: Is there a controversy? journal December 2018
Evaluating experimental and theoretical measures of protein conformational dynamics dissertation January 2016
New approaches towards the understanding of integral membrane proteins: A structural perspective on G protein-coupled receptors: Structural Perspective on G Protein-Coupled Receptors journal June 2017
Structural biology of G protein‐coupled receptor signaling complexes journal December 2018
Endosomal PTH Receptor Signaling Through cAMP and Its Consequence for Human Medicine book January 2017
Structures of Non-rhodopsin GPCRs Elucidated Through X-Ray Crystallography book January 2017
NMR Spectroscopy for the Characterization of GPCR Energy Landscapes book January 2017
Structure and Activation Mechanism of GPCRs book January 2019
Crystallization of Membrane Proteins: An Overview book January 2017
Current and Future Challenges in GPCR Drug Discovery book November 2017
Cell-Translocation Mechanisms of CPPs book June 2019
Allosteric modulation model of the mu opioid receptor by herkinorin, a potent not alkaloidal agonist journal March 2017
Molecular Mechanism of Biased Signaling in a Prototypical G-protein-coupled Receptor journal February 2020
Allosteric Modulation as a Unifying Mechanism for Receptor Function and Regulation journal August 2016
Molecular assembly of rhodopsin with G protein-coupled receptor kinases journal May 2017
Intracellular allosteric antagonism of the CCR9 receptor journal December 2016
Mechanism of intracellular allosteric β2AR antagonist revealed by X-ray crystal structure journal August 2017
Functional competence of a partially engaged GPCR–β-arrestin complex journal November 2016
Structural basis of ligand interaction with atypical chemokine receptor 3 journal January 2017
Biased signalling: from simple switches to allosteric microprocessors journal January 2018
Hitchhiking on the heptahelical highway: structure and function of 7TM receptor complexes journal April 2016
Nanodiscs for structural and functional studies of membrane proteins journal June 2016
Crystal structure of the human 5-HT1B serotonin receptor bound to an inverse agonist journal March 2018
GPCR signaling: conformational activation of arrestins journal July 2018
A complex structure of arrestin-2 bound to a G protein-coupled receptor journal November 2019
Structural basis of arrestin-3 activation and signaling journal November 2017
Development of an antibody fragment that stabilizes GPCR/G-protein complexes journal September 2018
Molecular mechanism of GPCR-mediated arrestin activation journal May 2018
Structural insights into G-protein-coupled receptor allostery journal July 2018
Allosteric mechanisms underlie GPCR signaling to SH3-domain proteins through arrestin journal August 2018
Structure of an allosteric modulator bound to the CB1 cannabinoid receptor journal October 2019
Computational design of G Protein-Coupled Receptor allosteric signal transductions journal December 2019
An online resource for GPCR structure determination and analysis journal January 2019
Time-resolved serial femtosecond crystallography at the European XFEL journal November 2019
Structure and dynamics of GPCR signaling complexes journal January 2018
Molecular mechanism of modulating arrestin conformation by GPCR phosphorylation journal June 2018
Molecular pharmacology of metabotropic receptors targeted by neuropsychiatric drugs journal July 2019
Structure of an endosomal signaling GPCR–G protein–β-arrestin megacomplex journal November 2019
Hydroxyethyl cellulose matrix applied to serial crystallography journal April 2017
Arrestin-1 engineering facilitates complex stabilization with native rhodopsin journal January 2019
Combinatorial allosteric modulation of agonist response in a self-interacting G-protein coupled receptor journal January 2020
Peptide mini-scaffold facilitates JNK3 activation in cells journal February 2016
Protein crystal screening and characterization for serial femtosecond nanocrystallography journal May 2016
Vibrational resonance, allostery, and activation in rhodopsin-like G protein-coupled receptors journal November 2016
Investigating allosteric effects on the functional dynamics of β2-adrenergic ternary complexes with enhanced-sampling simulations journal January 2017
G s protein peptidomimetics as allosteric modulators of the β 2 -adrenergic receptor journal January 2018
A comparative study of branched and linear mannitol-based amphiphiles on membrane protein stability journal January 2018
Vitamin E-based glycoside amphiphiles for membrane protein structural studies journal January 2018
Asymmetric maltose neopentyl glycol amphiphiles for a membrane protein study: effect of detergent asymmetricity on protein stability journal January 2019
GPCR structure and function relationship: identification of a biased apelin receptor mutant journal December 2018
Structural biology and structure–function relationships of membrane proteins journal December 2018
X-ray free electron laser: opportunities for drug discovery journal November 2017
The contribution of modern EPR to structural biology journal February 2018
Microfluidics: From crystallization to serial time-resolved crystallography journal May 2017
Evaluation of serial crystallographic structure determination within megahertz pulse trains journal November 2019
β-arrestin–biased signaling through the β 2 -adrenergic receptor promotes cardiomyocyte contraction journal June 2016
Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody journal July 2017
Ligand-specific conformational transitions and intracellular transport are required for atypical chemokine receptor 3–mediated chemokine scavenging journal November 2017
The β-Arrestins: Multifunctional Regulators of G Protein-coupled Receptors journal April 2016
Residues and residue pairs of evolutionary importance differentially direct signaling bias of D2 dopamine receptors journal November 2019
Cryo-EM structure of the native rhodopsin dimer in nanodiscs journal August 2019
Genetic code expansion and photocross-linking identify different β-arrestin binding modes to the angiotensin II type 1 receptor journal September 2019
Serial Femtosecond Crystallography journal November 2015
An outlook on using serial femtosecond crystallography in drug discovery journal May 2019
Watching proteins function with time-resolved x-ray crystallography journal August 2017
Short-wavelength free-electron laser sources and science: a review journal October 2017
Identification of the endocytic sorting signal recognized by the Art1-Rsp5 ubiquitin ligase complex journal December 2016
Sequence, Structure, and Expression of Opsins in the Monochromatic Stomatopod Squilla empusa journal April 2018
GPCRdb: an information system for G protein-coupled receptors journal November 2015
GPCRdb: an information system for G protein-coupled receptors journal December 2016
Melanopsin Carboxy-terminus Phosphorylation Plasticity and Bulk Negative Charge, not Strict Site Specificity, Achieves Phototransduction Deactivation journal January 2020
A complex structure of arrestin-2 bound to a G protein-coupled receptor journal October 2019
Ultrafast Dynamics of a Nucleobase Analogue Illuminated by a Short Intense X-ray Free Electron Laser Pulse journal June 2016
Linac Coherent Light Source: The first five years journal March 2016
X-ray free-electron laser: opportunities for drug discovery journal July 2018
Data processing pipeline for serial femtosecond crystallography at SACLA journal April 2016
Protein structure determination by single-wavelength anomalous diffraction phasing of X-ray free-electron laser data journal March 2016
XFELs for structure and dynamics in biology journal May 2017
Biological single-particle imaging using XFELs – towards the next resolution revolution journal October 2018
Fixed-target serial oscillation crystallography at room temperature journal February 2019
Fixed-target serial oscillation crystallography at room temperature text January 2019
Fixed-target serial oscillation crystallography at room temperature text January 2019
Toward G protein-coupled receptor structure-based drug design using X-ray lasers journal October 2019
In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures journal January 2016
Where is crystallography going? journal February 2018
Sample delivery for serial crystallography at free-electron lasers and synchrotrons journal January 2019
Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments journal October 2019
GPCRdb: the G protein-coupled receptor database - an introduction: GPCRdb: the GPCR database journal June 2016
Update on melatonin receptors: IUPHAR Review 20: Melatonin receptors journal August 2016
Allosteric modulation as a unifying mechanism for receptor function and regulation journal September 2017
Structural insights into melatonin receptors journal November 2019
Endo-lysosomal sorting of G-protein-coupled receptors by ubiquitin: Diverse pathways for G-protein-coupled receptor destruction and beyond journal November 2018
The Multiple Waves of Cannabinoid 1 Receptor Signaling journal June 2016
Chemokine Receptor Crystal Structures: What Can Be Learned from Them? journal July 2019
The Diverse Roles of Arrestin Scaffolds in G Protein–Coupled Receptor Signaling journal April 2017
Crystal structure of rhodopsin in complex with a mini-G o sheds light on the principles of G protein selectivity journal September 2018
Single-particle cryo-EM—How did it get here and where will it go journal August 2018
Molecular mechanism of biased signaling in a prototypical G protein–coupled receptor journal February 2020
CCR5 adopts three homodimeric conformations that control cell surface delivery journal May 2018
G protein–coupled receptor kinases (GRKs) orchestrate biased agonism at the β 2 -adrenergic receptor journal August 2018
A structural basis for how ligand binding site changes can allosterically regulate GPCR signaling and engender functional selectivity journal February 2020
G-Protein–Coupled Receptors in Heart Disease journal August 2018
Uncovering missing pieces: duplication and deletion history of arrestins in deuterostomes journal July 2017
Cross-communication between Gi and Gs in a G-protein-coupled receptor heterotetramer guided by a receptor C-terminal domain journal February 2018
Common and biased signaling pathways of the chemokine receptor CCR7 elicited by its ligands CCL19 and CCL21 in leukocytes journal January 2016
Accurate determination of segmented X-ray detector geometry journal January 2015
Structure-Based Sequence Alignment of the Transmembrane Domains of All Human GPCRs: Phylogenetic, Structural and Functional Implications journal March 2016
Molecular dynamics simulation of the follicle-stimulating hormone receptor. Understanding the conformational dynamics of receptor variants at positions N680 and D408 from in silico analysis journal November 2018
Critical role of the finger loop in arrestin binding to the receptors journal March 2019
Differential Phosphorylation Provides a Switch to Control How α-Arrestin Rod1 Down-regulates Mating Pheromone Response in Saccharomyces cerevisiae journal May 2016
C-edge loops of arrestin function as a membrane anchor text January 2017
Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody text January 2017
Common activation mechanism of class A GPCRs. journalarticle January 2019
Accurate determination of segmented X-ray detector geometry text January 2015
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography text January 2016
X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex text January 2016
Short-wavelength free-electron laser sources and science: a review text January 2017
Megahertz serial crystallography text January 2018
Demonstration of femtosecond X-ray pump X-ray probe diffraction on protein crystals text January 2018
Biological single-particle imaging using XFELs – towards the next resolution revolution text January 2018
Structure-Function Relationships of the Follicle-Stimulating Hormone Receptor journal November 2018
GPCR Signaling Regulation: The Role of GRKs and Arrestins journal February 2019
Cannabinoid CB1 and CB2 Receptor-Mediated Arrestin Translocation: Species, Subtype, and Agonist-Dependence journal April 2019
Recent Advances in Ultrafast Structural Techniques journal April 2019
Structural Biology and Structure–Function Relationships of Membrane Proteins journal March 2021
An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization journal July 2017
The XFEL Protein Crystallography: Developments and Perspectives journal July 2019
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography text January 2016
Crystal structure of rhodopsin in complex with a mini-Go sheds light on the principles of G protein selectivity text January 2018
Arrestin-1 engineering facilitates complex stabilization with native rhodopsin text January 2019
In vivo assembly and large-scale purification of a GPCR - Gα fusion with Gβγ, and characterization of the active complex text January 2019
Common activation mechanism of class A GPCRs journal December 2019

Similar Records

X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex
Journal Article · Tue Apr 12 00:00:00 EDT 2016 · Scientific Data · OSTI ID:1251413
A Model for the Signal Initiation Complex Between Arrestin-3 and the Src Family Kinase Fgr
Journal Article · Fri Dec 10 23:00:00 EST 2021 · Journal of Molecular Biology · OSTI ID:2538510
Crystal Packing Analysis of Rhodopsin Crystals
Journal Article · Tue Jul 10 00:00:00 EDT 2007 · J.Struc.Biol.158:455,2007 · OSTI ID:909779

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
G protein-coupled receptors
X-ray crystallography