Stability and performance of silica-based alkyl bonded-phase HPLC column packings with pH > 8 mobile phases
- Rockland Technologies, Inc., Newport, DE (United States)
Certain HPLC separations are best performed with high pH (pH >8) mobile phases where basic compounds may not be fully protonated. However, the traditional recommendation for silica-based HPLC column packings is that they not be used with mobile phases above pH 8 because of rapid packed-bed degradation by solubility of the silica support. Studies now have shown that packings with certain types of silica supports and bonded phases can be employed successfully for routine separations with mobile phases of at least pH 9. Column packing lifetime with higher pH mobile phases also can be extended by proper choice of buffer type. Silica support solubility (and ultimate column life) is significantly influenced by the nature of both the anions and cations used in the mobile phase buffer. Even at pH 7, some alkyl bonded phase packings show poor lifetime with higher concentrations of certain buffers, especially at elevated temperatures. Column temperature and buffer concentration also greatly affect silica support solubility and ultimate column lifetime. Findings from this study should assist in the development of more rugged HPLC methods for routine applications.
- OSTI ID:
- 126071
- Report Number(s):
- CONF-950402--
- Country of Publication:
- United States
- Language:
- English
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