Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin: Metabolic Tools for Enhanced Algal Fitness in the Prominent Order Prymnesiales (Haptophyceae)

Hovde, Blake T.; Deodato, Chloe R.; Hunsperger, Heather M.; Ryken, Scott A.; Yost, Will; Jha, Ramesh K.; Patterson, Johnathan; Monnat, Raymond J.; Barlow, Steven B.; Starkenburg, Shawn R.; Cattolico, Rose Ann; Richardson, Paul M.

doi:10.1371/journal.pgen.1005469

Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin: Metabolic Tools for Enhanced Algal Fitness in the Prominent Order Prymnesiales (Haptophyceae)

Journal Article · Wed Sep 23 00:00:00 EDT 2015 · PLoS Genetics

DOI:https://doi.org/10.1371/journal.pgen.1005469· OSTI ID:1259483

Hovde, Blake T. ^[1]; Deodato, Chloe R. ^[1]; Hunsperger, Heather M. ^[1]; Ryken, Scott A. ^[1]; Yost, Will ^[1]; Jha, Ramesh K. ^[2]; Patterson, Johnathan ^[1]; Monnat, Raymond J. ^[1]; Barlow, Steven B. ^[3]; Starkenburg, Shawn R. ^[2]; Cattolico, Rose Ann ^[1]; Richardson, Paul M. ^[4]

Univ. of Washington, Seattle, WA (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
San Diego State Univ., CA (United States)
Micro Trek Inc., Davis, CA (United States)

Haptophytes are recognized as seminal players in aquatic ecosystem function. These algae are important in global carbon sequestration, form destructive harmful blooms, and given their rich fatty acid content, serve as a highly nutritive food source to a broad range of eco-cohorts. Haptophyte dominance in both fresh and marine waters is supported by the mixotrophic nature of many taxa. Despite their importance the nuclear genome sequence of only one haptophyte, Emiliania huxleyi (Isochrysidales), is available. Here we report the draft genome sequence of Chrysochromulina tobin (Prymnesiales), and transcriptome data collected at seven time points over a 24-hour light/dark cycle. The nuclear genome of C. tobin is small (59 Mb), compact (~40% of the genome is protein coding) and encodes approximately 16,777 genes. Genes important to fatty acid synthesis, modification, and catabolism show distinct patterns of expression when monitored over the circadian photoperiod. The C. tobin genome harbors the first hybrid polyketide synthase/non-ribosomal peptide synthase gene complex reported for an algal species, and encodes potential anti-microbial peptides and proteins involved in multidrug and toxic compound extrusion. A new haptophyte xanthorhodopsin was also identified, together with two “red” RuBisCO activases that are shared across many algal lineages. In conclusion, the Chrysochromulina tobin genome sequence provides new information on the evolutionary history, ecology and economic importance of haptophytes.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: EE0003046

OSTI ID:: 1259483

Journal Information:: PLoS Genetics, Journal Name: PLoS Genetics Journal Issue: 9 Vol. 11; ISSN 1553-7404

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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