Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution

Yan, Hanfei; Nazaretski, Evgeny; Lauer, Kenneth R.; Huang, Xiaojing; Wagner, Ulrich; Rau, Christoph; Yusuf, Mohammed; Robinson, Ian K.; Kalbfleisch, Sebastian; Li, Li; Bouet, Nathalie; Zhou, Juan; Conley, Ray; Chu, Yong S.

doi:10.1038/srep20112

Title: Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution

Journal Article · Fri Feb 05 00:00:00 EST 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep20112· OSTI ID:1248377

Yan, Hanfei ^[1]; Nazaretski, Evgeny ^[1]; Lauer, Kenneth R. ^[1]; Huang, Xiaojing ^[1]; Wagner, Ulrich ^[2]; Rau, Christoph ^[2]; Yusuf, Mohammed ^[3]; Robinson, Ian K. ^[3]; Kalbfleisch, Sebastian ^[1]; Li, Li ^[1]; Bouet, Nathalie ^[1]; Zhou, Juan ^[1]; Conley, Ray ^[4]; Chu, Yong S. ^[1]

Brookhaven National Lab. (BNL), Upton, NY (United States)
Diamond Light Source Ltd., Oxfordshire (United Kingdom)
Univ. College London, London (United Kingdom); Rutherford Appleton Lab., Oxfordshire (United Kingdom)
Brookhaven National Lab. (BNL), Upton, NY (United States); Argonne National Lab. (ANL), Argonne, IL (United States)

Here, we developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1248377

Journal Information:: Scientific Reports, Vol. 6; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 41 works

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