Apparatus and method for nanoflow liquid jet and serial femtosecond x-ray protein crystallography

Bogan, Michael J.; Laksmono, Hartawan; Sierra, Raymond G.

Title: Apparatus and method for nanoflow liquid jet and serial femtosecond x-ray protein crystallography

Patent · Tue Mar 01 00:00:00 EST 2016

OSTI ID:1240690

Bogan, Michael J.; Laksmono, Hartawan; Sierra, Raymond G.

Techniques for nanoflow serial femtosecond x-ray protein crystallography include providing a sample fluid by mixing a plurality of a first target of interest with a carrier fluid and injecting the sample fluid into a vacuum chamber at a rate less than about 4 microliters per minute. In some embodiments, the carrier fluid has a viscosity greater than about 3 centipoise.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-76SF00515

Assignee:: The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA)

Patent Number(s):: 9,275,844

Application Number:: 13/896,303

OSTI ID:: 1240690

Resource Relation:: Patent File Date: 2013 May 16

Country of Publication:: United States

Language:: English

References (31)

Nano ES GEMMA and PDMA, new tools for the analysis of nanobioparticles—Protein complexes, lipoparticles, and viruses Allmaier, Günter; Laschober, Christian; Szymanski, Wladyslaw W. Journal of the American Society for Mass Spectrometry, Vol. 19, Issue 8, p. 1062-1068 https://doi.org/10.1016/j.jasms.2008.05.017	journal	August 2008
Energy-dispersive X-ray emission spectroscopy using an X-ray free-electron laser in a shot-by-shot mode Alonso-Mori, R.; Kern, J.; Gildea, R. J. Proceedings of the National Academy of Sciences, Vol. 109, Issue 47, p. 19103-19107 https://doi.org/10.1073/pnas.1211384109	journal	November 2012
Time-resolved protein nanocrystallography using an X-ray free-electron laser Aquila, Andrew; Hunter, Mark S.; Doak, R. Bruce Optics Express, Vol. 20, Issue 3 https://doi.org/10.1364/OE.20.002706	journal	January 2012
Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements Barty, Anton; Caleman, Carl; Aquila, Andrew Nature Photonics, Vol. 6, Issue 1 https://doi.org/10.1038/nphoton.2011.297	journal	December 2011
Non-destructive characterization and alignment of aerodynamically focused particle beams using single particle charge detection Benner, W. Henry; Bogan, Michael J.; Rohner, Urs Journal of Aerosol Science, Vol. 39, Issue 11, p. 917-928 https://doi.org/10.1016/j.jaerosci.2008.05.008	journal	November 2008
Single Particle X-ray Diffractive Imaging Bogan, Michael J.; Benner, W. Henry; Boutet, Sébastien Nano Letters, Vol. 8, Issue 1, p. 310-316 https://doi.org/10.1021/nl072728k	journal	January 2008
Femtosecond X-ray protein nanocrystallography Chapman, Henry N.; Fromme, Petra; Barty, Anton Nature, Vol. 470, Issue 7332, p. 73-77 https://doi.org/10.1038/nature09750	journal	February 2011
Gas dynamic virtual nozzle for generation of microscopic droplet streams DePonte, D. P.; Weierstall, U.; Schmidt, K. Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505 https://doi.org/10.1088/0022-3727/41/19/195505	journal	September 2008
SEM imaging of liquid jets DePonte, Daniel P.; Doak, R. B.; Hunter, Mark Micron, Vol. 40, Issue 4, p. 507-509 https://doi.org/10.1016/j.micron.2008.12.009	journal	June 2009
Sample injection for pulsed x-ray sources DePonte, Daniel P.; Nass, Karol; Stellato, Francesco SPIE Optics + Optoelectronics, SPIE Proceedings https://doi.org/10.1117/12.886780	conference	May 2011
X-ray Diffraction from Membrane Protein Nanocrystals Hunter, M. S.; DePonte, D. P.; Shapiro, D. A. Biophysical Journal, Vol. 100, Issue 1, p. 198-206 https://doi.org/10.1016/j.bpj.2010.10.049	journal	January 2011
Lipidic phase membrane protein serial femtosecond crystallography Johansson, Linda C.; Arnlund, David; White, Thomas A. Nature Methods, Vol. 9, Issue 3 https://doi.org/10.1038/nmeth.1867	journal	January 2012
Generation of Nonagglomerated Airborne Bacteriophage Particles Using an Electrospray Technique Jung, Jae Hee; Lee, Jung Eun; Kim, Sang Soo Analytical Chemistry, Vol. 81, Issue 8 https://doi.org/10.1021/ac802584z	journal	April 2009
Room temperature femtosecond X-ray diffraction of photosystem II microcrystals Kern, J.; Alonso-Mori, R.; Hellmich, J. Proceedings of the National Academy of Sciences, Vol. 109, Issue 25, p. 9721-9726 https://doi.org/10.1073/pnas.1204598109	journal	June 2012
Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature Kern, J.; Alonso-Mori, R.; Tran, R. Science, Vol. 340, Issue 6131 https://doi.org/10.1126/science.1234273	journal	February 2013
In vivo protein crystallization opens new routes in structural biology Koopmann, Rudolf; Cupelli, Karolina; Redecke, Lars Nature Methods, Vol. 9, Issue 3 https://doi.org/10.1038/nmeth.1859	journal	January 2012
Electrohydrodynamic spraying characteristics of glycerol solutions in vacuum Ku, Bon Ki; Kim, Sang Soo Journal of Electrostatics, Vol. 57, Issue 2, p. 109-128 https://doi.org/10.1016/S0304-3886(02)00106-7	journal	February 2003
Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser Lomb, Lukas; Barends, Thomas R. M.; Kassemeyer, Stephan Physical Review B, Vol. 84, Issue 21 https://doi.org/10.1103/PhysRevB.84.214111	journal	December 2011
Charge Reduction in Electrosprays: Slender Nanojets as Intermediates Marginean, Ioan; Znamenskiy, Vasiliy; Vertes, Akos The Journal of Physical Chemistry B, Vol. 110, Issue 12 https://doi.org/10.1021/jp055708k	journal	March 2006
Electrospray-assisted characterization and deposition of chlorosomes to fabricate a biomimetic light-harvesting device Modesto-Lopez, Luis B.; Thimsen, Elijah J.; Collins, Aaron M. Energy Environ. Sci., Vol. 3, Issue 2, p. 216-222 https://doi.org/10.1039/B914758F	journal	January 2010
Electrospray deposition of carbon nanotubes in vacuum O’Shea, James N.; Taylor, John B.; Swarbrick, Janine C. Nanotechnology, Vol. 18, Issue 3 https://doi.org/10.1088/0957-4484/18/3/035707	journal	January 2007
Protein Structures under Electrospray Conditions Patriksson, Alexandra; Marklund, Erik; van der Spoel, David Biochemistry, Vol. 46, Issue 4 https://doi.org/10.1021/bi061182y	journal	January 2007
Powder diffraction from a continuous microjet of submicrometer protein crystals Shapiro, D. A.; Chapman, H. N.; DePonte, D. Journal of Synchrotron Radiation, Vol. 15, Issue 6 https://doi.org/10.1107/S0909049508024151	journal	October 2008
Nanoflow electrospinning serial femtosecond crystallography Sierra, Raymond G.; Laksmono, Hartawan; Kern, Jan Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 11, p. 1584-1587 https://doi.org/10.1107/S0907444912038152	journal	October 2012
Electrospray deposition in vacuum Swarbrick, Janine C.; Taylor, J. Ben; O'Shea, James N. Applied Surface Science, Vol. 252, Issue 15, p. 5622-5626 https://doi.org/10.1016/j.apsusc.2005.12.025	journal	May 2006
Shrinking droplets in electrospray ionization and their influence on chemical equilibria Wortmann, Arno; Kistler-Momotova, Anna; Zenobi, Renato Journal of the American Society for Mass Spectrometry, Vol. 18, Issue 3, p. 385-393 https://doi.org/10.1016/j.jasms.2006.10.010	journal	March 2007
Mass spectrometry of solution and apparatus therefor Takada, Yasuaki; Sakairi, Minoru; Hirabayashi, Atsumu https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6252225 US Patent Document 6,252,225	patent	June 2001
Method and apparatus for electrospray ionization Fenn, John B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6297499 US Patent Document 6,297,499	patent	October 2001
Ionization chamber for atmospheric pressure ionization mass spectrometry Park, Melvin A.; Wang, Houle; Laukien, Frank https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7315020 US Patent Document 7,315,020	patent	January 2008
Contiguous capillary electrospray sources and analytical devices Janini, George; Issaq, Haleem J.; Veenstra, Timothy D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7544932 US Patent Document 7,544,932	patent	June 2009
Method and apparatus for nano-capillary/micro electrospray for use in liquid chromatography-mass spectrometry Zhu, Yixin; Nugent, Kerry D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8227750 US Patent Document 8,227,750	patent	July 2012

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