Suppression of conformational heterogeneity at a protein–protein interface

Deis, Lindsay N.; Wu, Qinglin; Wang, You; Qi, Yang; Daniels, Kyle G.; Zhou, Pei; Oas, Terrence G.

doi:10.1073/pnas.1424724112

Suppression of conformational heterogeneity at a protein–protein interface

Journal Article · Wed Jul 08 00:00:00 EDT 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1424724112· OSTI ID:1239417

Deis, Lindsay N. ^[1]; Wu, Qinglin ^[1]; Wang, You ^[1]; Qi, Yang ^[1]; Daniels, Kyle G. ^[1]; Zhou, Pei ^[1]; Oas, Terrence G. ^[1]

Duke Univ., Durham, NC (United States)

Staphylococcal protein A (SpA) is an important virulence factor fromStaphylococcus aureusresponsible for the bacterium’s evasion of the host immune system. SpA includes five small three-helix–bundle domains that can each bind with high affinity to many host proteins such as antibodies. The interaction between a SpA domain and the F_c fragment of IgG was partially elucidated previously in the crystal structure 1FC2. Although informative, the previous structure was not properly folded and left many substantial questions unanswered, such as a detailed description of the tertiary structure of SpA domains in complex with F_c and the structural changes that take place upon binding. Here we report the 2.3-Å structure of a fully folded SpA domain in complex with F_c. Our structure indicates that there are extensive structural rearrangements necessary for binding F_c, including a general reduction in SpA conformational heterogeneity, freezing out of polyrotameric interfacial residues, and displacement of a SpA side chain by an F_c side chain in a molecular-recognition pocket. Such a loss of conformational heterogeneity upon formation of the protein–protein interface may occur when SpA binds its multiple binding partners. As a result, suppression of conformational heterogeneity may be an important structural paradigm in functionally plastic proteins.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE

Grant/Contract Number:: W-31109-ENG-38

OSTI ID:: 1239417

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 29 Vol. 112; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
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Suppression of conformational heterogeneity at a protein–protein interface

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