Cooperative control between AtRGS1 and AtHXK1 in a WD40-repeat protein pathway in Arabidopsis thaliana

Huang, Jian -Ping; Tunc-Ozdemir, Meral; Chang, Ying; Jones, Alan M.

doi:10.3389/fpls.2015.00851

Cooperative control between AtRGS1 and AtHXK1 in a WD40-repeat protein pathway in Arabidopsis thaliana

Journal Article · Tue Oct 13 04:00:00 EDT 2015 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2015.00851· OSTI ID:1239293

Huang, Jian -Ping ^[1]; Tunc-Ozdemir, Meral ^[2]; Chang, Ying ^[3]; Jones, Alan M. ^[2]

Northeast Agricultural Univ., Harbin (China); Univ. of North Carolina, Chapel Hill, NC (United States); None
Univ. of North Carolina, Chapel Hill, NC (United States)
Northeast Agricultural Univ., Harbin (China)

HEXOKINASE 1 (AtHXK1) and Regulator of G-protein Signaling 1 (AtRGS1) pathways, mediate D-glucose signaling in Arabidopsis. However, it is not known the degree, if any, that these pathways overlap and how. We show modest signaling crosstalk between these pathways, albeit complex with both epistatic interactions and additive effects that may be indirect. The action of HXK1 on AtRGS1 signaling lies downstream of the primary step in G protein-mediated sugar signaling in which the WD-repeat protein, AGB1, is the propelling signaling element. RHIP1, a previously unknown protein predicted here to have a 3-stranded helical structure, interacts with both AtRGS1 and AtHXK1 in planta and is required for some glucose-regulated gene expression, providing a physical connection between these two proteins in sugar signaling. The rhip1 null mutant displays similar seedling growth phenotypes as rgs1-2 in response to glucose, further suggesting a role for RHIP1 in glucose signaling. Lastly, glucose signaling is a complex hierarchical relationship which is specific to the target gene and sugar phenotype and suggests that there are two glycolysis-independent glucose signaling sensors: AtRGS1 and AtHXK1 that weakly communicate with each other via feed-back and feed-forward loops to fine tune the response to glucose.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of North Carolina, Chapel Hill, NC (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-05ER15671

OSTI ID:: 1239293

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 6; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cooperative control between AtRGS1 and AtHXK1 in a WD40-repeat protein pathway in Arabidopsis thaliana

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