Differential regulation of G protein signaling in Arabidopsis through two distinct pathways that internalize AtRGS1

Watkins, Justin M.; Ross-Elliott, Timothy J.; Shan, Xiaoyi; Lou, Fei; Dreyer, Bernd; Tunc-Ozdemir, Meral; Jia, Haiyan; Yang, Jing; Oliveira, Celio Cabral; Wu, Luguang; Trusov, Yuri; Schwochert, Timothy D.; Krysan, Patrick; Jones, Alan M.

doi:10.1126/scisignal.abe4090

Differential regulation of G protein signaling in Arabidopsis through two distinct pathways that internalize AtRGS1

Journal Article · Tue Aug 10 00:00:00 EDT 2021 · Science Signaling

DOI:https://doi.org/10.1126/scisignal.abe4090· OSTI ID:1812769

^[1]; ^[1]; Shan, Xiaoyi ^[1]; Lou, Fei ^[1]; ^[1]; Tunc-Ozdemir, Meral ^[1]; Jia, Haiyan ^[1]; Yang, Jing ^[1]; ^[2]; Wu, Luguang ^[3]; ^[3]; Schwochert, Timothy D. ^[4]; ^[5]; ^[6]

Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Department of Biochemistry and Molecular Biology/Bioagro, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
School of Agriculture and Food Science, University of Queensland, St. Lucia, Queensland Q4072, Australia.
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Department of Horticulture, University of Wisconsin Madison, Madison, WI 53706, USA.
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

In animals, endocytosis of a seven-transmembrane GPCR is mediated by arrestins to propagate or arrest cytoplasmic G protein–mediated signaling, depending on the bias of the receptor or ligand, which determines how much one transduction pathway is used compared to another. In Arabidopsis thaliana , GPCRs are not required for G protein–coupled signaling because the heterotrimeric G protein complex spontaneously exchanges nucleotide. Instead, the seven-transmembrane protein AtRGS1 modulates G protein signaling through ligand-dependent endocytosis, which initiates derepression of signaling without the involvement of canonical arrestins. Here, we found that endocytosis of AtRGS1 initiated from two separate pools of plasma membrane: sterol-dependent domains and a clathrin-accessible neighborhood, each with a select set of discriminators, activators, and candidate arrestin-like adaptors. Ligand identity (either the pathogen-associated molecular pattern flg22 or the sugar glucose) determined the origin of AtRGS1 endocytosis. Different trafficking origins and trajectories led to different cellular outcomes. Thus, in this system, compartmentation with its associated signalosome architecture drives biased signaling.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-05ER15671

OSTI ID:: 1812769

Alternate ID(s):: OSTI ID: 1849582

Journal Information:: Science Signaling, Journal Name: Science Signaling Journal Issue: 695 Vol. 14; ISSN 1945-0877

Publisher:: American Association for the Advancement of Science (AAAS)Copyright Statement

Country of Publication:: United States

Language:: English

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