Tracking single-particle rotation during macrophage uptake

Sanchez, Lucero; Patton, Paul; Anthony, Stephen Michael; Yi, Yi; Yu, Yan

doi:10.1039/C5SM00893J

Tracking single-particle rotation during macrophage uptake

Journal Article · Wed Jun 10 00:00:00 EDT 2015 · Soft Matter

DOI:https://doi.org/10.1039/C5SM00893J· OSTI ID:1235366

Sanchez, Lucero ^[1]; Patton, Paul ^[1]; Anthony, Stephen Michael ^[2]; Yi, Yi ^[1]; Yu, Yan ^[1]

Indiana Univ., Bloomington, IN (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

We investigated the rotational dynamics of single microparticles during their internalization by macrophage cells. The microparticles used were triblock patchy particles that display two fluorescent patches on their two poles. The optical anisotropy made it possible to directly visualize and quantify the orientation and rotation of the particles. We show that particles exhibit a mixture of fast and slow rotation as they are uptaken by macrophages and transiently undergo directional rotation during their entry into the cell. As a result, the size of the particles and the surface presentation of ligands exerted a negligible influence on this heterogeneity of particle rotation.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: Indiana Univ., Bloomington, IN (United States)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1235366

Report Number(s):: SAND--2015-3290J; 583488

Journal Information:: Soft Matter, Journal Name: Soft Matter Journal Issue: 26 Vol. 11; ISSN SMOABF; ISSN 1744-683X

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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