A transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of intracellular survival

Bent, Zachary W.; Poorey, Kunal; Brazel, David M.; LaBauve, Annette E.; Sinha, Anupama; Curtis, Deanna Joy; House, Samantha E.; Tew, Karen E.; Hamblin, Rachelle Y.; Williams, Kelly Porter; Branda, Steven S.; Young, Glenn M.; Meagher, Robert J.

doi:10.1128/IAI.02922-14

A transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of intracellular survival

Journal Article · Mon Apr 20 00:00:00 EDT 2015 · Infection and Immunity

DOI:https://doi.org/10.1128/IAI.02922-14· OSTI ID:1235333

Bent, Zachary W. ^[1]; Poorey, Kunal ^[1]; Brazel, David M. ^[1]; LaBauve, Annette E. ^[1]; Sinha, Anupama ^[1]; Curtis, Deanna Joy ^[1]; House, Samantha E. ^[1]; Tew, Karen E. ^[1]; Hamblin, Rachelle Y. ^[1]; Williams, Kelly Porter ^[1]; Branda, Steven S. ^[1]; Young, Glenn M. ^[2]; Meagher, Robert J. ^[1]

Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Univ. of California, Davis, CA (United States)

Yersinia enterocolitica is typically considered an extracellular pathogen; however, during the course of an infection, a significant number of bacteria are stably maintained within host cell vacuoles. Little is known about this population and the role it plays during an infection. To address this question and to elucidate the spatially and temporally dynamic gene expression patterns of Y. enterocoliticabiovar 1B through the course of an in vitro infection, transcriptome sequencing and differential gene expression analysis of bacteria infecting murine macrophage cells were performed under four distinct conditions. Bacteria were first grown in a nutrient-rich medium at 26°C to establish a baseline of gene expression that is unrelated to infection. The transcriptomes of these bacteria were then compared to bacteria grown in a conditioned cell culture medium at 37°C to identify genes that were differentially expressed in response to the increased temperature and medium but not in response to host cells. Infections were then performed, and the transcriptomes of bacteria found on the extracellular surface and intracellular compartments were analyzed individually. The upregulated genes revealed potential roles for a variety of systems in promoting intracellular virulence, including the Ysa type III secretion system, the Yts2 type II secretion system, and the Tad pilus. It was further determined that mutants of each of these systems had decreased virulence while infecting macrophages. Overall, these results reveal the complete set of genes expressed by Y. enterocolitica in response to infection and provide the groundwork for future virulence studies.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1235333

Report Number(s):: SAND--2014-3732J; 516866

Journal Information:: Infection and Immunity, Journal Name: Infection and Immunity Journal Issue: 7 Vol. 83; ISSN 0019-9567

Country of Publication:: United States

Language:: English

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