Glycine activated ion channel subunits encoded by ctenophore glutamate receptor genes

Alberstein, Robert; Grey, Richard; Zimmet, Austin; Simmons, David K.; Mayer, Mark L.

doi:10.1073/pnas.1513771112

Glycine activated ion channel subunits encoded by ctenophore glutamate receptor genes

Journal Article · Mon Oct 12 00:00:00 EDT 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1513771112· OSTI ID:1228098

Alberstein, Robert ^[1]; Grey, Richard ^[1]; Zimmet, Austin ^[1]; Simmons, David K. ^[2]; Mayer, Mark L. ^[1]

National Inst. of Health (NIH), Bethesda, MD (United States). National Inst. of Child Health and Human Development. Lab. of Cellular and Molecular Neurophysiology
Univ. of Florida, St. Augustine, FL (United States). The Whitney Lab. for Marine Bioscience

Recent genome projects for ctenophores have revealed the presence of numerous ionotropic glutamate receptors (iGluRs) in Mnemiopsis leidyi and Pleurobrachia bachei, among our earliest metazoan ancestors. Sequence alignments and phylogenetic analysis show that these form a distinct clade from the well-characterized AMPA, kainate, and NMDA iGluR subtypes found in vertebrates. Although annotated as glutamate and kainate receptors, crystal structures of the ML032222a and PbiGluR3 ligand-binding domains (LBDs) reveal endogenous glycine in the binding pocket, whereas ligand-binding assays show that glycine binds with nanomolar affinity; biochemical assays and structural analysis establish that glutamate is occluded from the binding cavity. Further analysis reveals ctenophore-specific features, such as an interdomain Arg-Glu salt bridge, present only in subunits that bind glycine, but also a conserved disulfide in loop 1 of the LBD that is found in all vertebrate NMDA but not AMPA or kainate receptors. In this paper, we hypothesize that ctenophore iGluRs are related to an early ancestor of NMDA receptors, suggesting a common evolutionary path for ctenophores and bilaterian species, and finally suggest that future work should consider both glycine and glutamate as candidate neurotransmitters in ctenophore species.

View Accepted Manuscript (DOE)

Research Organization:: National Inst. of Health (NIH), Bethesda, MD (United States)

Sponsoring Organization:: National Aeronautics and Space Administration (NASA) (United States); National Inst. of Health (NIH) (United States); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Contributing Organization:: Univ. of Florida, St. Augustine, FL (United States)

Grant/Contract Number:: W-31109-ENG-38

OSTI ID:: 1228098

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 44 Vol. 112; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
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Glycine activated ion channel subunits encoded by ctenophore glutamate receptor genes

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