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Title: Polypeptide having cellobiohydrolase activity and uses thereof

Patent ·
OSTI ID:1215543

The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Research Organization:
DSM IP ASSETS B.V., Heerlen (Netherlands)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-08GO18079
Assignee:
DSM IP ASSETS B.V. (Heerlen, NL)
Patent Number(s):
9,133,448
Application Number:
13/578,223
OSTI ID:
1215543
Resource Relation:
Patent File Date: 2011 Feb 11
Country of Publication:
United States
Language:
English

References (7)

Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them patent January 2012
Method for treating cellulosic material and CBHII/CEL6A enzymes useful therein patent November 2013
Modified Family 6 Glycosidases with Altered Substrate Specificity patent-application January 2010
Talaromyces Transformants patent-application November 2012
Identification of functionally important amino acids in the cellulose-binding domain of Trichoderma reesei cellobiohydrolase I : Effect of amino acid substitutions on CBHt CBD journal June 1995
Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 Å resolution journal January 1999
Correlation between cellulose binding and activity of cellulose-binding domain mutants of Humicola grisea cellobiohydrolase 1 journal December 2007