Nano-immunoassay with improved performance for detection of cancer biomarkers

Krasnoslobodtsev, Alexey V.; Torres, Maria P.; Kaur, Sukhwinder; Vlassiouk, Ivan V.; Lipert, Robert J.; Jain, Maneesh; Batra, Surinder K.; Lyubchenko, Yuri L.

doi:10.1016/j.nano.2014.08.012

Nano-immunoassay with improved performance for detection of cancer biomarkers

Journal Article · Thu Jan 01 00:00:00 EST 2015 · Nanomedicine: Nanotechnologoy, Biology, and Medicine

DOI:https://doi.org/10.1016/j.nano.2014.08.012· OSTI ID:1214460

Krasnoslobodtsev, Alexey V. ^[1]; Torres, Maria P. ^[1]; Kaur, Sukhwinder ^[1]; Vlassiouk, Ivan V. ^[2]; Lipert, Robert J. ^[3]; Jain, Maneesh ^[1]; Batra, Surinder K. ^[1]; Lyubchenko, Yuri L. ^[1]

Univ. of Nebraska Medical Center, Omaha, NE (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Iowa State Univ., Ames, IA (United States)

Nano-immunoassay utilizing surface-enhanced Raman scattering (SERS) effect is a promising analytical technique for the early detection of cancer. In its current standing the assay is capable of discriminating samples of healthy individuals from samples of pancreatic cancer patients. Further improvements in sensitivity and reproducibility will extend practical applications of the SERS-based detection platforms to wider range of problems. In this report, we discuss several strategies designed to improve performance of the SERS-based detection system. We demonstrate that reproducibility of the platform is enhanced by using atomically smooth mica surface as a template for preparation of capture surface in SERS sandwich immunoassay. Furthermore, the assay's stability and sensitivity can be further improved by using either polymer or graphene monolayer as a thin protective layer applied on top of the assay addresses. The protective layer renders the signal to be more stable against photo-induced damage and carbonaceous contamination.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1214460

Journal Information:: Nanomedicine: Nanotechnologoy, Biology, and Medicine, Journal Name: Nanomedicine: Nanotechnologoy, Biology, and Medicine Journal Issue: 1 Vol. 11; ISSN 1549-9634

Country of Publication:: United States

Language:: English

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Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate Israelsen, Nathan D.; Wooley, Donald; Hanson, Cynthia Journal of Biological Engineering, Vol. 10, Issue 1 https://doi.org/10.1186/s13036-015-0023-y	journal	January 2016
Surface-Enhanced Raman Scattering-Based Immunoassay Technologies for Detection of Disease Biomarkers Smolsky, Joseph; Kaur, Sukhwinder; Hayashi, Chihiro Biosensors, Vol. 7, Issue 4 https://doi.org/10.3390/bios7010007	journal	January 2017
Application of Gold Nanoparticle to Plasmonic Biosensors Lee, Jin-Ho; Cho, Hyeon-Yeol; Choi, Hye International Journal of Molecular Sciences, Vol. 19, Issue 7 https://doi.org/10.3390/ijms19072021	journal	July 2018
Gold Nanoparticle Based Platforms for Circulating Cancer Marker Detection Huang, Xiaohua; O'Connor, Ryan; Kwizera, Elyahb Allie Nanotheranostics, Vol. 1, Issue 1 https://doi.org/10.7150/ntno.18216	journal	January 2017
Engineering State‐of‐the‐Art Plasmonic Nanomaterials for SERS‐Based Clinical Liquid Biopsy Applications Wang, Jing; Koo, Kevin M.; Wang, Yuling Advanced Science, Vol. 6, Issue 23 https://doi.org/10.1002/advs.201900730	journal	September 2019
Multiplex in vitro detection using SERS Laing, Stacey; Gracie, Kirsten; Faulds, Karen Chemical Society Reviews, Vol. 45, Issue 7 https://doi.org/10.1039/c5cs00644a	journal	January 2016
Raman spectroscopy using plasmonic and carbon-based nanoparticles for cancer detection, diagnosis, and treatment guidance.Part 1: Diagnosis Darrigues, Emilie; Nima, Zeid A.; Majeed, Waqar Drug Metabolism Reviews, Vol. 49, Issue 2 https://doi.org/10.1080/03602532.2017.1302465	journal	April 2017
A Review on Surface-Enhanced Raman Scattering Pilot, Robert; Signorini, Raffaella; Durante, Christian Biosensors, Vol. 9, Issue 2, p. 57 https://doi.org/10.3390/bios9020057	journal	April 2019

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