Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams

Coquelle, Nicolas; Brewster, Aaron S.; Kapp, Ulrike; Shilova, Anastasya; Weinhausen, Britta; Burghammer, Manfred; Colletier, Jacques -Philippe

doi:10.1107/S1399004715004514

Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams

Journal Article · Sat Apr 25 04:00:00 EDT 2015 · Acta Crystallographica. Section D: Biological Crystallography (Online)

DOI:https://doi.org/10.1107/S1399004715004514· OSTI ID:1213447

Coquelle, Nicolas ^[1]; Brewster, Aaron S. ^[2]; Kapp, Ulrike ^[3]; Shilova, Anastasya ^[3]; Weinhausen, Britta ^[3]; Burghammer, Manfred ^[4]; Colletier, Jacques -Philippe ^[1]

Universite´ Grenoble Alpes, Genoble (France); CNRS, Grenoble (France); CEA, Genable (France)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
European Synchrotron Radiation Facility, Grenoble (France)
European Synchrotron Radiation Facility, Grenoble (France); Ghent Univ., Ghent (Belgium)

High-resolution structural information was obtained from lysozyme microcrystals (20 µm in the largest dimension) using raster-scanning serial protein crystallography on micro- and nano-focused beamlines at the ESRF. Data were collected at room temperature (RT) from crystals sandwiched between two silicon nitride wafers, thereby preventing their drying, while limiting background scattering and sample consumption. In order to identify crystal hits, new multi-processing and GUI-driven Python-based pre-analysis software was developed, named NanoPeakCell, that was able to read data from a variety of crystallographic image formats. Further data processing was carried out using CrystFEL, and the resultant structures were refined to 1.7 Å resolution. The data demonstrate the feasibility of RT raster-scanning serial micro- and nano-protein crystallography at synchrotrons and validate it as an alternative approach for the collection of high-resolution structural data from micro-sized crystals. Advantages of the proposed approach are its thriftiness, its handling-free nature, the reduced amount of sample required, the adjustable hit rate, the high indexing rate and the minimization of background scattering.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1213447

Alternate ID(s):: OSTI ID: 22351153

Journal Information:: Acta Crystallographica. Section D: Biological Crystallography (Online), Journal Name: Acta Crystallographica. Section D: Biological Crystallography (Online) Journal Issue: 5 Vol. 71; ISSN 1399-0047; ISSN ABCRE6

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Fixed-target serial oscillation crystallography at room temperature Wierman, Jennifer L.; Paré-Labrosse, Olivier; Sarracini, Antoine International Union of Crystallography (IUCr) https://doi.org/10.1107/s2052252519001453/mf5030sup2.mp4	text	January 2019
High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source Martin-Garcia, Jose M.; Zhu, Lan; Mendez, Derek IUCrJ, Vol. 6, Issue 3 https://doi.org/10.1107/s205225251900263x	journal	April 2019
High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source Martin-Garcia, Jose M.; Zhu, Lan; Mendez, Derek International Union of Crystallography (IUCr) https://doi.org/10.1107/s205225251900263x/ti5014sup1.pdf	text	January 2019
Synchrotron microcrystal native-SAD phasing at a low energy Guo, Gongrui; Zhu, Ping; Fuchs, Martin R. IUCrJ, Vol. 6, Issue 4 https://doi.org/10.1107/s2052252519004536	journal	May 2019
Carbonic anhydrase II microcrystals suitable for XFEL studies Lomelino, Carrie L.; Kim, Jin Kyun; Lee, Cheol Acta Crystallographica Section F Structural Biology Communications, Vol. 74, Issue 6 https://doi.org/10.1107/s2053230x18006118	journal	May 2018
EIGER detector: application in macromolecular crystallography Casanas, Arnau; Warshamanage, Rangana; Finke, Aaron D. Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 9 https://doi.org/10.1107/s2059798316012304	journal	August 2016
Low-dose fixed-target serial synchrotron crystallography Owen, Robin L.; Axford, Danny; Sherrell, Darren A. Acta Crystallographica Section D Structural Biology, Vol. 73, Issue 4, p. 373-378 https://doi.org/10.1107/s2059798317002996	journal	March 2017
Crystallography on a chip – without the chip: sheet-on-sheet sandwich Doak, R. Bruce; Nass Kovacs, Gabriela; Gorel, Alexander Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 10 https://doi.org/10.1107/s2059798318011634	journal	October 2018
Sample delivery for serial crystallography at free-electron lasers and synchrotrons Grünbein, Marie Luise; Nass Kovacs, Gabriela Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s205979831801567x	journal	January 2019
ZOO : an automatic data-collection system for high-throughput structure analysis in protein microcrystallography Hirata, Kunio; Yamashita, Keitaro; Ueno, Go Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017795	journal	January 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017953	journal	February 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s2059798318017953/ba5296sup1.pdf	text	January 2019
Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments Andersson, Rebecka; Safari, Cecilia; Båth, Petra Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 10 https://doi.org/10.1107/s2059798319012695	journal	October 2019
A guide to sample delivery systems for serial crystallography Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai The FEBS Journal, Vol. 286, Issue 22 https://doi.org/10.1111/febs.15099	journal	August 2019
Time-resolved structural studies with serial crystallography: A new light on retinal proteins Panneels, Valérie; Wu, Wenting; Tsai, Ching-Ju Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2015-04290	text	January 2015
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography Nogly, Przemyslaw; Panneels, Valerie; Nelson, Garrett Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2016-03927	text	January 2016
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography Nogly, Przemyslaw; Panneels, Valérie; Nelson, Garrett ETH Zurich https://doi.org/10.3929/ethz-b-000119795	text	January 2016
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons Weinert, Tobias; Olieric, Natacha; Cheng, Robert ETH Zurich https://doi.org/10.3929/ethz-b-000191628	text	January 2017

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