Production of carrier-peptide conjugates using chemically reactive unnatural amino acids

Young, Travis; Schultz, Peter G.

Production of carrier-peptide conjugates using chemically reactive unnatural amino acids

Patent · Tue Aug 18 00:00:00 EDT 2015

OSTI ID:1210885

Young, Travis; Schultz, Peter G.

Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.

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Research Organization:: The Scripps Research Institute, La Jolla, CA (United States)

Sponsoring Organization:: USDOE

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Number(s):: 9,109,022

Application Number:: 14/065,111

OSTI ID:: 1210885

Country of Publication:: United States

Language:: English

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