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Title: Production of carrier-peptide conjugates using chemically reactive unnatural amino acids

Patent ·
OSTI ID:1111109

Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.

Research Organization:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-00ER46051
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,609,383
Application Number:
13/133,629
OSTI ID:
1111109
Country of Publication:
United States
Language:
English

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