Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Schultz, Peter G.; Alfonta, Lital; Chittuluru, Johnathan R.; Deiters, Alexander; Groff, Dan; Summerer, Daniel; Tsao, Meng -Lin; Wang, Jiangyun; Wu, Ning; Xie, Jianming; Zeng, Huaqiang; Seyedsayamdost, Mohammad; Turner, James

Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Patent · Tue Aug 11 00:00:00 EDT 2015

OSTI ID:1209477

Schultz, Peter G.; Alfonta, Lital; Chittuluru, Johnathan R.; Deiters, Alexander; Groff, Dan; Summerer, Daniel; Tsao, Meng -Lin; Wang, Jiangyun; Wu, Ning; Xie, Jianming; Zeng, Huaqiang; Seyedsayamdost, Mohammad; Turner, James

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

Research Organization:: The Scripps Research Institute, La Jolla, CA (United States)

Sponsoring Organization:: USDOE

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Number(s):: 9,102,932

Application Number:: 13/492,296

OSTI ID:: 1209477

Country of Publication:: United States

Language:: English

References (12)

The Genetic Incorporation of a Distance Probe into Proteins in Escherichia coli Tsao, Meng-Lin; Summerer, Daniel; Ryu, Youngha Journal of the American Chemical Society, Vol. 128, Issue 14 https://doi.org/10.1021/ja058262u	journal	April 2006
A genetically encoded fluorescent amino acid Summerer, D.; Chen, S.; Wu, N. Proceedings of the National Academy of Sciences, Vol. 103, Issue 26, p. 9785-9789 https://doi.org/10.1073/pnas.0603965103	journal	June 2006
Expanding the Genetic Code of Escherichia coli Wang, L.; Brock, A.; Herberich, B. Science, Vol. 292, Issue 5516, p. 498-500 https://doi.org/10.1126/science.1060077	journal	April 2001
An Expanded Eukaryotic Genetic Code Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher Science, Vol. 301, Issue 5635, p. 964-967 https://doi.org/10.1126/science.1084772	journal	August 2003
The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination Xie, Jianming; Wang, Lei; Wu, Ning Nature Biotechnology, Vol. 22, Issue 10 https://doi.org/10.1038/nbt1013	journal	September 2004
Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo Liu, D. R.; Magliery, T. J.; Pastrnak, M. Proceedings of the National Academy of Sciences, Vol. 94, Issue 19 https://doi.org/10.1073/pnas.94.19.10092	journal	September 1997
A Genetically Encoded Photocaged Tyrosine Deiters, Alexander; Groff, Dan; Ryu, Youngha Angewandte Chemie International Edition, Vol. 45, Issue 17, p. 2728-2731 https://doi.org/10.1002/anie.200600264	journal	April 2006
An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli Santoro, S. W. Nucleic Acids Research, Vol. 31, Issue 23 https://doi.org/10.1093/nar/gkg903	journal	December 2003
Genetic introduction of a diketone-containing amino acid into proteins Zeng, Huaqiang; Xie, Jianming; Schultz, Peter G. Bioorganic & Medicinal Chemistry Letters, Vol. 16, Issue 20, p. 5356-5359 https://doi.org/10.1016/j.bmcl.2006.07.094	journal	October 2006
Adding amino acids to the genetic repertoire Xie, Jianming; Schultz, Peter G. Current Opinion in Chemical Biology, Vol. 9, Issue 6, p. 548-554 https://doi.org/10.1016/j.cbpa.2005.10.011	journal	December 2005
Expanding the Genetic Code Wang, Lei; Schultz, Peter G. Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66 https://doi.org/10.1002/anie.200460627	journal	January 2005
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli Chin, J. W.; Martin, A. B.; King, D. S. Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024 https://doi.org/10.1073/pnas.172226299	journal	August 2002

Similar Records

Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Patent · Tue Jul 10 00:00:00 EDT 2012 · OSTI ID:1176455

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Patent · Tue Sep 07 00:00:00 EDT 2010 · OSTI ID:1014688

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Patent · Tue Mar 12 00:00:00 EDT 2013 · OSTI ID:1083058

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Citation Formats

References (12)

Similar Records

Related Subjects