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Title: RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes

Journal Article · · Scientific Reports
DOI:https://doi.org/10.1038/srep08365· OSTI ID:1194128
 [1];  [1];  [2];  [3];  [4];  [5];  [6];  [4];  [4];  [4];  [7];  [8];  [1];  [4];  [7];  [6]
  1. Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of Chicago, Chicago, IL (United States)
  2. Fellowship for Interpretation of Genomes, Burr Ridge, IL (United States)
  3. Argonne National Lab. (ANL), Argonne, IL (United States); San Diego State Univ., San Diego, CA (United States)
  4. Argonne National Lab. (ANL), Argonne, IL (United States); Fellowship for Interpretation of Genomes, Burr Ridge, IL (United States)
  5. University of Illinois at Urbana-Champaign, Urbana, IL (US)
  6. Univ. of Chicago, Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
  7. Virginia Tech Univ., Blacksburg, VA (United States)
  8. USDA-ARS Lab. at Cold Spring Harbor Lab., NY (United States)

The RAST (Rapid Annotation using Subsystem Technology) annotation engine was built in 2008 to annotate bacterial and archaeal genomes. It works by offering a standard software pipeline for identifying genomic features (i.e., protein-encoding genes and RNA) and annotating their functions. Recently, in order to make RAST a more useful research tool and to keep pace with advancements in bioinformatics, it has become desirable to build a version of RAST that is both customizable and extensible. In this paper, we describe the RAST tool kit (RASTtk), a modular version of RAST that enables researchers to build custom annotation pipelines. RASTtk offers a choice of software for identifying and annotating genomic features as well as the ability to add custom features to an annotation job. RASTtk also accommodates the batch submission of genomes and the ability to customize annotation protocols for batch submissions. This is the first major software restructuring of RAST since its inception.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1194128
Journal Information:
Scientific Reports, Vol. 5, Issue 1; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 1279 works
Citation information provided by
Web of Science

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Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput journal June 2019
Hydrolytic Capabilities as a Key to Environmental Success: Chitinolytic and Cellulolytic Acidobacteria From Acidic Sub-arctic Soils and Boreal Peatlands journal November 2018
Production of hemolysin BL by Bacillus cereus group isolates of dairy origin is associated with whole-genome phylogenetic clade journal August 2016
Draft Genome Sequences of Four Antibiotic-Resistant Salmonella Strains Isolated from Australian Red Meat Animal Species journal August 2019
Interrogation of a live-attenuated enterotoxigenic Escherichia coli vaccine highlights features unique to wild-type infection journal August 2019
Complete Genome Sequence of “Candidatus Syntrophocurvum alkaliphilum” Strain B(2M), Obtained from the Metagenome of a Salt-Tolerant Alkaliphilic Anaerobic Syntrophic Butyrate-Degrading Consortium journal February 2020
Transcriptomic profiling of Streptococcus pyogenes M1T1 strain in a mouse model of necrotizing fasciitis posted_content April 2019
A machine learning-based service for estimating quality of genomes using PATRIC journal October 2019
Resilience of small intestinal beneficial bacteria to the toxicity of soybean oil fatty acids journal March 2018
New Genus Fibralongavirus in Siphoviridae Phages of Staphylococcus pseudintermedius journal December 2019
Pan-Genomic and Polymorphic Driven Prediction of Antibiotic Resistance in Elizabethkingia journal July 2019
Complete Genome Sequences of Two Escherichia Phages Isolated from Wastewater in Finland journal May 2018
Complete Genome Sequence of Lelliottia nimipressuralis Type Strain SGAir0187, Isolated from Tropical Air Collected in Singapore journal May 2018
Production of multiple bacteriocins, including the novel bacteriocin gassericin M, by Lactobacillus gasseri LM19, a strain isolated from human milk journal November 2019
A metagenomic window into the 2-km-deep terrestrial subsurface aquifer revealed multiple pathways of organic matter decomposition journal August 2018
Mobilizable antibiotic resistance genes are present in dust microbial communities journal January 2020
Draft Genome Sequence of Plant Growth-Promoting Endophytic Microbacterium hydrothermale BPSAC84, Isolated from the Medicinal Plant Mirabilis jalapa journal May 2019
Strain typing and characterization of virulence genes in clinical Staphylococcus aureus isolates from Kenya posted_content August 2018
Complete Genome Sequence of Agrococcus sp. Strain SGAir0287, Isolated from Tropical Air Collected in Singapore journal August 2019
Comprehensive analysis of draft genomes of two closely related pseudomonas syringae phylogroup 2b strains infecting mono- and dicotyledon host plants journal December 2016
The draft genomes of Elizabethkingia anophelis of equine origin are genetically similar to three isolates from human clinical specimens journal July 2018
Litorilituus lipolyticus sp. nov., isolated from intertidal sand of the Yellow Sea in China, and emended description of Colwellia asteriadis journal November 2019
SXT/R391 integrative and conjugative elements in Proteus species reveal abundant genetic diversity and multidrug resistance journal November 2016
Extended-spectrum beta-lactamases and plasmid diversity in urinary isolates of Escherichia coli in Croatia: a nation-wide, multicentric, retrospective study journal January 2020
Complete Genome Sequence of Pseudomonas stutzeri Type Strain SGAir0442, Isolated from Singapore Air Samples journal May 2018
Molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates with focus on antimicrobial resistance journal September 2019
Characterization of Salmonella Dublin isolated from bovine and human hosts journal October 2019
Multi-omics reveal various potential antimonate reductases from phylogenetically diverse microorganisms journal September 2019
Genomics of Aerobic Photoheterotrophs in Wheat Phyllosphere Reveals Divergent Evolutionary Patterns of Photosynthetic Genes in Methylobacterium spp. journal September 2019
Data on draft genome sequence of Bacillus sp. strain VKPM B-3276 isolated from Culex pipiens larvae journal June 2019
Complete genome sequence of a natural compounds producer, Streptomyces violaceus S21 journal June 2017
Comparative genomics of Salmonella enterica serovar Enteritidis ST-11 isolated in Uruguay reveals lineages associated with particular epidemiological traits journal February 2020
Complete Genome Sequence of the Biocontrol Agent Bacillus velezensis UFLA258 and Its Comparison with Related Species: Diversity within the Commons journal October 2019
Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, “Silenusvirus” journal August 2019
Genome Sequence of Elizabethkingia anophelis Strain EaAs1, Isolated from the Asian Malaria Mosquito Anopheles stephensi journal April 2016
Draft Genome Sequences of Four Pseudomonas aeruginosa Isolates Obtained from Patients with Chronic Obstructive Pulmonary Disease journal June 2017
Draft Genome Sequence of Aliiroseovarius crassostreae CV919-312, the Causative Agent of Roseovarius Oyster Disease (Formerly Juvenile Oyster Disease) journal April 2016
Draft Genome Sequence of the Tacrolimus-Producing Bacterium Streptomyces tsukubaensis F601 journal May 2017
Complete Genome Sequence of Melissococcus plutonius DAT561, a Strain That Shows an Unusual Growth Profile, Obtained by PacBio Sequencing journal June 2018
Draft Genome Sequence of the Emerging Bivalve Pathogen Vibrio tubiashii subsp. europaeus journal August 2016
Genome Sequences of Klebsiella variicola Isolates from Dairy Animals with Bovine Mastitis from Newfoundland, Canada journal October 2015
Draft Genome Sequence of Lactobacillus plantarum Strain SNU.Lp177 from Pig Feces in South Korea journal October 2015
Draft Genome Sequence of Hypervirulent and Vaccine Candidate Streptococcus suis Strain SC19 journal January 2017
Complete Genome Sequence of the Triclosan- and Multidrug-Resistant Pseudomonas aeruginosa Strain B10W Isolated from Municipal Wastewater journal January 2017
Genome Sequence of Megasphaera cerevisiae NSB1, a Bacterium Isolated from a Canning Line and Able To Grow in Beer with High Alcohol Content journal February 2017
Draft Genome Sequences of Two Vibrio splendidus Strains, Isolated from Seagrass Sediment journal February 2016
Draft Genome Sequence of Bacillus subtilis TLO3, Isolated from Olive Tree Rhizosphere Soil journal September 2018
Genomic Alterations of Staphylococcus aureus ATCC 25923 after Prolonged Passage in the Laboratory journal November 2018
Draft Genome Sequence of Serratia sp. 1D1416 journal January 2019
Genome Sequence of Paracoccus sp. JM45, a Bacterial Strain Isolated from a Marine Sponge with a Dual Quorum Sensing Inhibition Activity journal January 2019
Metabolic Network Analysis and Metatranscriptomics Reveal Auxotrophies and Nutrient Sources of the Cosmopolitan Freshwater Microbial Lineage acI journal August 2017
The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6 journal March 2017
Evolution of Antibiotic Synthesis Gene Clusters in the Streptomyces globisporus TFH56, Isolated from Tomato Flower journal June 2019
Why Close a Bacterial Genome? The Plasmid of Alteromonas Macleodii HOT1A3 is a Vector for Inter-Specific Transfer of a Flexible Genomic Island journal March 2016
Respiratory Ammonification of Nitrate Coupled to Anaerobic Oxidation of Elemental Sulfur in Deep-Sea Autotrophic Thermophilic Bacteria journal January 2017
Replicon-Based Typing of IncI-Complex Plasmids, and Comparative Genomics Analysis of IncIγ/K1 Plasmids journal January 2019
Microevolution and Adaptive Strategy of Psychrophilic Species Flavobacterium bomense sp. nov. Isolated From Glaciers journal May 2019
Genome of a Novel Bacterium “Candidatus Jettenia ecosi” Reconstructed From the Metagenome of an Anammox Bioreactor journal October 2019
Escherichia coli ST302: Genomic Analysis of Virulence Potential and Antimicrobial Resistance Mediated by Mobile Genetic Elements journal January 2020
Capsular Switching and ICE Transformation Occurred in Human Streptococcus agalactiae ST19 With High Pathogenicity to Fish journal November 2018
Sequencing and Analysis of the Genome of Propionibacterium freudenreichii T82 Strain: Importance for Industry journal February 2020
Biotechnological Potential of Cold Adapted Pseudoalteromonas spp. Isolated from ‘Deep Sea’ Sponges journal June 2017
Genetic Screening and Expression Analysis of Psychrophilic Bacillus spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat journal September 2019
Genome Sequence and Analysis ofPeptoclostridium difficileStrain ZJCDC-S82 journal January 2016

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