The RAST server : rapid annotations using subsystems technology.
The number of prokaryotic genome sequences becoming available is growing steadily and is growing faster than our ability to accurately annotate them. We describe a fully automated service for annotating bacterial and archaeal genomes. The service identifies protein-encoding, rRNA and tRNA genes, assigns functions to the genes, predicts which subsystems are represented in the genome, uses this information to reconstruct the metabolic network and makes the output easily downloadable for the user. In addition, the annotated genome can be browsed in an environment that supports comparative analysis with the annotated genomes maintained in the SEED environment. The service normally makes the annotated genome available within 12-24 hours of submission, but ultimately the quality of such a service will be judged in terms of accuracy, consistency, and completeness of the produced annotations. We summarize our attempts to address these issues and discuss plans for incrementally enhancing the service. By providing accurate, rapid annotation freely to the community we have created an important community resource. The service has now been utilized by over 120 external users annotating over 350 distinct genomes.
- Research Organization:
- Argonne National Laboratory (ANL)
- Sponsoring Organization:
- SC; NIH
- DOE Contract Number:
- AC02-06CH11357
- OSTI ID:
- 927765
- Report Number(s):
- ANL/MCS/JA-61372
- Journal Information:
- BMC Genomics, Journal Name: BMC Genomics Journal Issue: Feb. 8. 2008 Vol. 9
- Country of Publication:
- United States
- Language:
- ENGLISH
Similar Records
RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes
RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes
The metagenomics RAST server - a public resource for the automatic phylogenetic and functional analysis of metagenomes.
Journal Article
·
Mon Feb 09 23:00:00 EST 2015
· Scientific Reports
·
OSTI ID:1391665
RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes
Journal Article
·
Mon Feb 09 19:00:00 EST 2015
· Scientific Reports
·
OSTI ID:1194128
The metagenomics RAST server - a public resource for the automatic phylogenetic and functional analysis of metagenomes.
Journal Article
·
Fri Sep 19 00:00:00 EDT 2008
· BMC Bioinform.
·
OSTI ID:942107