Nucleic acid encoding a self-assembling split-fluorescent protein system

Waldo, Geoffrey S.; Cabantous, Stephanie

Nucleic acid encoding a self-assembling split-fluorescent protein system

Patent · Tue Jul 14 00:00:00 EDT 2015

OSTI ID:1193372

Waldo, Geoffrey S.; Cabantous, Stephanie

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

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Research Organization:: Los Alamos National Security, LLC, Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-98ER62647

Assignee:: Los Alamos National Security, LLC (Los Alamos, NM)

Patent Number(s):: 9,081,014

Application Number:: 14/166,770

OSTI ID:: 1193372

Country of Publication:: United States

Language:: English

References (35)

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