Nucleic acid encoding a self-assembling split-fluorescent protein system

Waldo, Geoffrey S; Cabantous, Stephanie

Nucleic acid encoding a self-assembling split-fluorescent protein system

Patent · 07 June 2011

OSTI ID:1019114

Waldo, Geoffrey S ^[1]; Cabantous, Stephanie ^[2]

Santa Fe, NM
Los Alamos, NM

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

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Research Organization:: Los Alamos National Security, LLC (Los Alamos, NM)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-98ER62647; W-7405-ENG-36

Assignee:: Los Alamos National Security, LLC (Los Alamos, NM)

Patent Number(s):: 7,955,821

Application Number:: US Patent Application 10/973,693

OSTI ID:: 1019114

Country of Publication:: United States

Language:: English

References (29)

Seeing what was unseen: New analytical methods for molecular imaging Umezawa, Yoshio The Chemical Record, Vol. 3, Issue 1 https://doi.org/10.1002/tcr.10049	journal	January 2003
Major colour patterns of reef-building corals are due to a family of GFP-like proteins Dove, S. G.; Hoegh-Guldberg, O.; Ranganathan, S. Coral Reefs, Vol. 19, Issue 3 https://doi.org/10.1007/PL00006956	journal	January 2001
Mutants of Discosoma red fluorescent protein with a GFP-like chromophore Wiehler, Jens; von Hummel, Julia; Steipe, Boris FEBS Letters, Vol. 487, Issue 3, p. 384-389 https://doi.org/10.1016/S0014-5793(00)02365-6	journal	January 2001
GFP-like chromoproteins as a source of far-red fluorescent proteins Gurskaya, Nadya G.; Fradkov, Arkady F.; Terskikh, Alexey FEBS Letters, Vol. 507, Issue 1, p. 16-20 https://doi.org/10.1016/S0014-5793(01)02930-1	journal	September 2001
Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation Hu, Chang-Deng; Chinenov, Yurii; Kerppola, Tom K. Molecular Cell, Vol. 9, Issue 4, p. 789-798 https://doi.org/10.1016/S1097-2765(02)00496-3	journal	April 2002
Combinatorial Marking of Cells and Organelles with Reconstituted Fluorescent Proteins Zhang, Shifang; Ma, Charles; Chalfie, Martin Cell, Vol. 119, Issue 1, p. 137-144 https://doi.org/10.1016/j.cell.2004.09.012	journal	October 2004
A Fluorescent Indicator for Detecting Protein−Protein Interactions in Vivo Based on Protein Splicing Ozawa, Takeaki; Nogami, Satoru; Sato, Moritoshi Analytical Chemistry, Vol. 72, Issue 21 https://doi.org/10.1021/ac000617z	journal	November 2000
Protein Splicing-Based Reconstitution of Split Green Fluorescent Protein for Monitoring Protein−Protein Interactions in Bacteria: Improved Sensitivity and Reduced Screening Time Ozawa, Takeaki; Takeuchi, Mizue; Kaihara, Asami Analytical Chemistry, Vol. 73, Issue 24 https://doi.org/10.1021/ac010717k	journal	December 2001
Green Fluorescent Protein (GFP): Applications, Structure, and Related Photophysical Behavior Zimmer, Marc Chemical Reviews, Vol. 102, Issue 3 https://doi.org/10.1021/cr010142r	journal	March 2002
Rapid protein-folding assay using green fluorescent protein Waldo, Geoffrey S.; Standish, Blake M.; Berendzen, Joel Nature Biotechnology, Vol. 17, Issue 7, p. 691-695 https://doi.org/10.1038/10904	journal	July 1999
Fluorescent proteins from nonbioluminescent Anthozoa species Matz, Mikhail V.; Fradkov, Arkady F.; Labas, Yulii A. Nature Biotechnology, Vol. 17, Issue 10 https://doi.org/10.1038/13657	journal	October 1999
Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein Wigley, W. Christian; Stidham, Rhesa D.; Smith, Nathan M. Nature Biotechnology, Vol. 19, Issue 2 https://doi.org/10.1038/84389	journal	February 2001
Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling Crameri, Andreas; Whitehorn, Erik A.; Tate, Emily Nature Biotechnology, Vol. 14, Issue 3 https://doi.org/10.1038/nbt0396-315	journal	March 1996
The molecular structure of green fluorescent protein Yang, Fan; Moss, Larry G.; Phillips, George N. Nature Biotechnology, Vol. 14, Issue 10 https://doi.org/10.1038/nbt1096-1246	journal	October 1996
Creating new fluorescent probes for cell biology Zhang, Jin; Campbell, Robert E.; Ting, Alice Y. Nature Reviews Molecular Cell Biology, Vol. 3, Issue 12 https://doi.org/10.1038/nrm976	journal	December 2002
Structures and technology for biologists Terwilliger, Thomas C. Nature Structural & Molecular Biology, Vol. 11, Issue 4 https://doi.org/10.1038/nsmb0404-296	journal	April 2004
A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria) Wiedenmann, J.; Schenk, A.; Rocker, C. Proceedings of the National Academy of Sciences, Vol. 99, Issue 18, p. 11646-11651 https://doi.org/10.1073/pnas.182157199	journal	August 2002
Identification of different emitting species in the red fluorescent protein DsRed by means of ensemble and single-molecule spectroscopy Cotlet, M.; Hofkens, J.; Habuchi, S. Proceedings of the National Academy of Sciences, Vol. 98, Issue 25, p. 14398-14403 https://doi.org/10.1073/pnas.251532698	journal	November 2001
Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments Pelletier, J. N.; Campbell-Valois, F. - X.; Michnick, S. W. Proceedings of the National Academy of Sciences, Vol. 95, Issue 21 https://doi.org/10.1073/pnas.95.21.12141	journal	October 1998
Circular permutation and receptor insertion within green fluorescent proteins Baird, G. S.; Zacharias, D. A.; Tsien, R. Y. Proceedings of the National Academy of Sciences, Vol. 96, Issue 20 https://doi.org/10.1073/pnas.96.20.11241	journal	September 1999
Natural Animal Coloration Can Be Determined by a Nonfluorescent Green Fluorescent Protein Homolog Lukyanov, Konstantin A.; Fradkov, Arkady F.; Gurskaya, Nadya G. Journal of Biological Chemistry, Vol. 275, Issue 34 https://doi.org/10.1074/jbc.C000338200	journal	June 2000
The 2.0-Å Crystal Structure of eqFP611, a Far Red Fluorescent Protein from the Sea Anemone Entacmaea quadricolor Petersen, Jan; Wilmann, Pascal G.; Beddoe, Travis Journal of Biological Chemistry, Vol. 278, Issue 45 https://doi.org/10.1074/jbc.M307896200	journal	August 2003
Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements Lutz, R. Nucleic Acids Research, Vol. 25, Issue 6, p. 1203-1210 https://doi.org/10.1093/nar/25.6.1203	journal	March 1997
The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral Beddoe, Travis; Ling, Michael; Dove, Sophie Acta Crystallographica Section D Biological Crystallography, Vol. 59, Issue 3, p. 597-599 https://doi.org/10.1107/S0907444902023466	journal	February 2003
Testing the role of chain connectivity on the stability and structure of dihydrofolate reductase from E. coli : Fragment complementation and circular permutation reveal stable, alternatively folded forms Smith, Virginia F.; Matthews, C. Robert Protein Science, Vol. 10, Issue 1 https://doi.org/10.1110/ps.26601	journal	January 2001
Crystal Structure of the Aequorea victoria Green Fluorescent Protein Orm , M.; Cubitt, A. B.; Kallio, K. Science, Vol. 273, Issue 5280 https://doi.org/10.1126/science.273.5280.1392	journal	September 1996
"Fluorescent Timer": Protein That Changes Color with Time Terskikh, A. Science, Vol. 290, Issue 5496 https://doi.org/10.1126/science.290.5496.1585	journal	November 2000
The Green Fluorescent Protein Tsien, Roger Y. Annual Review of Biochemistry, Vol. 67, Issue 1, p. 509-544 https://doi.org/10.1146/annurev.biochem.67.1.509	journal	June 1998
Interconversion of Anthozoa GFP-like fluorescent and non-fluorescent proteins by mutagenesis Bulina, Maria E.; Chudakov, Dmitry M.; Mudrik, Nikolay N. BMC Biochemistry, Vol. 3, Issue 1 https://doi.org/10.1186/1471-2091-3-7	journal	April 2002

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