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Title: Comparison of standard acute toxicity tests with rapid-screening toxicity tests

Abstract

This study compared the relative sensitivity of five inexpensive, rapid toxicity tests to the sensitivity of five standard aquatic acute toxicity tests through literature review and testing. The rapid toxicity tests utilized organisms that require little culturing or handling prior to testing: a freshwater rotifer (Branchionus ccalyciflorus); brine shrimp (Artemia salina); lettuce (Lactuca sativa); and two microbial tests (Photo bacterium phosphoreum - Microtox test, and a mixture of bacterial species - the polytox test). Standard acute toxicity test species included water fleas (Daphnia magna and Ceriadaphnta dubia), green algae (Setenastrum capricarnutum), fathead minnows (Pimephalespromelas), and mysid shrimp (Mysidopsis bahia). Sensitivity comparisons between rapid and standard acute toxicity tests were based on LC5O/EC50 data from 11 test chemicals. Individually, the lettuce and rotifer tests ranked most similar in sensitivity to the standard tests, while Microtox fell just outside the range of sensitivities represented by the group of standard acute toxicity tests. The brine shrimp and Polytox tests were one or more orders of magnitude different from the standard acute toxicity tests for most compounds. The lettuce, rotifer, and Microtox tests could be used as a battery for preliminary toxicity screening of chemicals. Further evaluation of complex real-world environmental samples is recommended.

Authors:
; ; ;
Publication Date:
Research Org.:
Army Biomedical Research and Development Lab., Fort Detrick, MD (United States)
OSTI Identifier:
118064
Report Number(s):
AD-A-294311/6/XAB
TRN: 52751351
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 1995
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; TOXICITY; TESTING

Citation Formats

Toussaint, M.W., Shedd, T.R., VanDerSchal, W.H., and Leather, G.R. Comparison of standard acute toxicity tests with rapid-screening toxicity tests. United States: N. p., 1995. Web.
Toussaint, M.W., Shedd, T.R., VanDerSchal, W.H., & Leather, G.R. Comparison of standard acute toxicity tests with rapid-screening toxicity tests. United States.
Toussaint, M.W., Shedd, T.R., VanDerSchal, W.H., and Leather, G.R. Sun . "Comparison of standard acute toxicity tests with rapid-screening toxicity tests". United States. doi:.
@article{osti_118064,
title = {Comparison of standard acute toxicity tests with rapid-screening toxicity tests},
author = {Toussaint, M.W. and Shedd, T.R. and VanDerSchal, W.H. and Leather, G.R.},
abstractNote = {This study compared the relative sensitivity of five inexpensive, rapid toxicity tests to the sensitivity of five standard aquatic acute toxicity tests through literature review and testing. The rapid toxicity tests utilized organisms that require little culturing or handling prior to testing: a freshwater rotifer (Branchionus ccalyciflorus); brine shrimp (Artemia salina); lettuce (Lactuca sativa); and two microbial tests (Photo bacterium phosphoreum - Microtox test, and a mixture of bacterial species - the polytox test). Standard acute toxicity test species included water fleas (Daphnia magna and Ceriadaphnta dubia), green algae (Setenastrum capricarnutum), fathead minnows (Pimephalespromelas), and mysid shrimp (Mysidopsis bahia). Sensitivity comparisons between rapid and standard acute toxicity tests were based on LC5O/EC50 data from 11 test chemicals. Individually, the lettuce and rotifer tests ranked most similar in sensitivity to the standard tests, while Microtox fell just outside the range of sensitivities represented by the group of standard acute toxicity tests. The brine shrimp and Polytox tests were one or more orders of magnitude different from the standard acute toxicity tests for most compounds. The lettuce, rotifer, and Microtox tests could be used as a battery for preliminary toxicity screening of chemicals. Further evaluation of complex real-world environmental samples is recommended.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Oct 01 00:00:00 EDT 1995},
month = {Sun Oct 01 00:00:00 EDT 1995}
}

Technical Report:
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