Devices, systems, and methods for microscale isoelectric fractionation

Sommer, Gregory J; Hatch, Anson V; Wang, Ying-Chih; Singh, Anup K

Devices, systems, and methods for microscale isoelectric fractionation

Patent · 14 April 2015

OSTI ID:1178015

Sommer, Gregory J; Hatch, Anson V; Wang, Ying-Chih; Singh, Anup K

Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.

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Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia Corporation (Albuquerque, NM)

Patent Number(s):: 9,005,417

Application Number:: 12/243,817

OSTI ID:: 1178015

Country of Publication:: United States

Language:: English

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