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Title: Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis

Abstract

Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.

Inventors:
; ; ;
Publication Date:
Research Org.:
SNL-A (Sandia National Laboratories, Albuquerque, NM (United States))
Sponsoring Org.:
USDOE
OSTI Identifier:
1082456
Patent Number(s):
8,329,016
Application Number:
12/551,047
Assignee:
Sandia Corporation (Albuquerque, NM)
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Sommer, Gregory J., Hatch, Anson V., Singh, Anup K., and Wang, Ying-Chih. Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis. United States: N. p., 2012. Web.
Sommer, Gregory J., Hatch, Anson V., Singh, Anup K., & Wang, Ying-Chih. Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis. United States.
Sommer, Gregory J., Hatch, Anson V., Singh, Anup K., and Wang, Ying-Chih. Tue . "Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis". United States. https://www.osti.gov/servlets/purl/1082456.
@article{osti_1082456,
title = {Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis},
author = {Sommer, Gregory J. and Hatch, Anson V. and Singh, Anup K. and Wang, Ying-Chih},
abstractNote = {Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {12}
}

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Works referenced in this record:

Integration of isoelectric focusing with multi-channel gel electrophoresis by using microfluidic pseudo-valves
journal, January 2007

  • Das, Champak; Zhang, Jiyou; Denslow, Nancy D.
  • Lab on a Chip, Vol. 7, Issue 12
  • DOI: 10.1039/b712794d

On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008

  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g