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Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production

Patent ·
OSTI ID:1176384
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A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism. It also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.

Research Organization:
E. I. du Pont de Nemours and Company, Wilmington, DE (United States); Alliance for Sustainable Energy LLC, Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-03GO13146
Assignee:
E. I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (Golden, CO)
Patent Number(s):
7,741,119
Application Number:
11/862,566
OSTI ID:
1176384
Country of Publication:
United States
Language:
English

References (17)

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Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production. journal January 1986
Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade journal January 2003
Site-specific and directional gene replacement mediated by Cre recombinase journal October 2000
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Characterization of a High-Productivity Recombinant Strain of Zymomonas mobilis for Ethanol Production from Glucose/Xylose Mixtures journal January 2000
Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection. journal January 1994
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002

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09 BIOMASS FUELS
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