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Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas

Patent ·
OSTI ID:1176383
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Production of ethanol using a strain of xylose-utilizing Zymomonas with a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.

Research Organization:
E. I. du Pont de Nemours and Company, Wilmington, DE (United States); Alliance for Sustainable Energy LLC, Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-03GO13146
Assignee:
E. I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (Golden, CO)
Patent Number(s):
7,741,084
Application Number:
11/862,736
OSTI ID:
1176383
Country of Publication:
United States
Language:
English

References (17)

Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains journal December 1992
Rapid ethanol production from sucrose without by-product formation journal September 1993
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis journal January 1995
Export of the periplasmic NADP-containing glucose-fructose oxidoreductase of Zymomonas mobilis journal July 1996
Bacteriophage P1 cre gene and its regulatory region journal January 1986
Nuclear Magnetic Resonance Studies of Acetic Acid Inhibition of Rec Zymomonas mobilis ZM4(pZB5) book January 2000
The Effect of Glucose on High-Level Xylose Fermentations by Recombinant Zymomonas in Batch and Fed-Batch Fermentations book January 1999
d-Xylose (d-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728. Purification and properties journal July 1991
Bacteriophage P1 site-specific recombination journal August 1981
Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production. journal January 1986
Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade journal January 2003
Site-specific and directional gene replacement mediated by Cre recombinase journal October 2000
Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant journal September 1992
Kinetic and Nuclear Magnetic Resonance Studies of Xylose Metabolism by Recombinant Zymomonas mobilisZM4(pZB5) journal January 2000
Characterization of a High-Productivity Recombinant Strain of Zymomonas mobilis for Ethanol Production from Glucose/Xylose Mixtures journal January 2000
Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection. journal January 1994
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002

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Related Subjects

09 BIOMASS FUELS
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