Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor

Burg, John S.; Ingram, Jessica R.; Venkatakrishnan, A. J.; Jude, Kevin M.; Dukkipati, Abhiram; Feinberg, Evan N.; Angelini, Alessandro; Waghray, Deepa; Dror, Ron O.; Ploegh, Hidde L.; Garcia, K. Christopher

doi:10.1126/science.aaa5026

Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor

Journal Article · Wed Mar 04 23:00:00 EST 2015 · Science

DOI:https://doi.org/10.1126/science.aaa5026· OSTI ID:1172427

Burg, John S.; Ingram, Jessica R.; Venkatakrishnan, A. J.; Jude, Kevin M.; Dukkipati, Abhiram; Feinberg, Evan N.; Angelini, Alessandro; Waghray, Deepa; Dror, Ron O.; Ploegh, Hidde L.; Garcia, K. Christopher ^[1]

Stanford

Chemokines are small proteins that function as immune modulators through activation of chemokine G protein-coupled receptors (GPCRs). Several viruses also encode chemokines and chemokine receptors to subvert the host immune response. How protein ligands activate GPCRs remains unknown. We report the crystal structure at 2.9 angstrom resolution of the human cytomegalovirus GPCR US28 in complex with the chemokine domain of human CX3CL1 (fractalkine). The globular body of CX3CL1 is perched on top of the US28 extracellular vestibule, whereas its amino terminus projects into the central core of US28. The transmembrane helices of US28 adopt an active-state-like conformation. Atomic-level simulations suggest that the agonist-independent activity of US28 may be due to an amino acid network evolved in the viral GPCR to destabilize the receptor’s inactive state.

View Accepted Manuscript (DOE)

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: NIHOTHERHHMI

OSTI ID:: 1172427

Journal Information:: Science, Journal Name: Science Journal Issue: 03, 2015 Vol. 347

Country of Publication:: United States

Language:: ENGLISH

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Latency-associated expression of human cytomegalovirus US28 attenuates cell attenuates cell signalling pathways to maintain latent infection Sinclair, John; Krishna, Benjamin; Poole, Emma Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.38836	text	January 2017
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