Genotoxicity of vanadium tetraoxide (V{sub 2}O{sub 4}) in human lymphocyte cultures

Journal Article · Wed Nov 01 04:00:00 EST 1995 · Environmental and Molecular Mutagenesis

OSTI ID:115145

Roldan-Reyes, E; Hernandez-Sanchez, E; Altamirano-Lozano, M

The oxy-cation vanadyl (VO{sup 2+}, +4 oxidation state) is one of the most stable ions in the first row of transition elements; it forms strong anionic, cationic, and neutral complexes with various types of ligands. From this behavior an interaction with a variety of biological functional groups can be predicted. However, vanadyl is most unstable at physiological pH and it is oxidized rapidly by air oxygen to form vanadate (+5 oxidation state). When vanadate enters the cell interior it is converted back to vanadyl by metabolism. In the present study were analyzed the cytotoxic and genotoxic effects of vanadium tetraoxide (V{sub 2}O{sub 4}). Lymphocytes cultures were incubated for 48 h at 37{degrees} C. 24 h. after initiation vanadium tetraoxide was added at concentrations of 4, 6, 8 {mu}g/ml. For each concentration and each experiment a minimum of 100 well-spread first-division metaphases were analyzed for structural and numerical chromosomal aberrations (SCA and NCA), 100 metaphases were analyzed for satellite associations (SA), and 1000 cells were analyzed for mitotic index (MI). Relative to control, tetroxide vanadium decreased the mitotic index (0.93% vs. 0.69 y 0.70% at concentrations of 4 and 8 {mu}g/ml). The frequency of structural chromosomal aberrations was increased (2.97, 2.66 and 3.93% in all concentrations vs. 0.51% to control), gap frequency were increased (3.96% for 4 {mu}g/ml and 1.22% for 8 {mu}g/ml vs 0.0% to control). The total cells with satellite associations were increased at all concentrations (71.55, 73.33, and 80.70% vs. 45.38% to control). The mean of satellite association cell were augmented at concentrations of 6 and 8 {mu}g/ml relative to control (1.52 and 1.64 vs. 1.37). Mean of chromosome satellite association only increased to high concentration (8{mu}g/ml) (3.76 vs. 3.21 to control). Results indicates the vanadium tetraoxide may be considered as potentially cytotoxic and genotoxic agent.

• OSTI ID: 115145
• Report Number(s): CONF-9503160--; CNN: Grant 500303; Grant IN-202593
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.25 Vol. 25; ISSN EMMUEG; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
CHROMOSOMAL ABERRATIONS
GENETIC EFFECTS
HUMAN CHROMOSOMES
LYMPHOCYTES
TOXICITY
VANADIUM COMPOUNDS