Dose-response of x-ray-induced anaphase aberrations in the mitotic root tip chromosomes of allium

Journal Article · Wed Nov 01 04:00:00 EST 1995 · Environmental and Molecular Mutagenesis

OSTI ID:115137

Ma, T H; Lee, K H; Kong, M S ^[1]

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1. Western Illinois Univ., Macomb, IL (United States); and others

A simplified Allium root mitotic chromosome aberration assay by using only the aberrant anaphases (fragments, laggards and bridges) as the end-points were developed by Rank and Nielsen (1993) for screening water soluble chemicals and complex mixtures. A dose-response curve was established by Meir et al., (1994) using a known clastogen, 4-nitroquinolene-N-oxide between the dose range of 0.1-0.5 ug/ml. In order to further validate this assay for clastogen detection, a series of X-ray dose response experiments was carried out. Allium roots were germinated in tapwater for 48 h and treated with a series of 10, 20, 30, 40, 50, 60 R (80 Kvp, 5 ma, dose rate 60 R/min) dosages. After an 18 hr recovery time, the root tips were hydrolyzed in 45% acetic and 1 N HC1 acid (9:1 ratio) solution under 50{degrees} C for 5 min and stained with aceto-carmine. Each of the data points were derived from scoring 7-10 slides (15-50 anaphases/slide). The corrrelation coefficient, slope and intercept values of the dose-response curve are: 0.954, 0.515 and 1.155 respectively.

• OSTI ID: 115137
• Report Number(s): CONF-9503160--
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.25 Vol. 25; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
CHROMOSOMAL ABERRATIONS
DOSE-RESPONSE RELATIONSHIPS
GENETIC RADIATION EFFECTS
ONIONS
X RADIATION