Alteration of pulmonary macrophage intracellular pH following inhalation exposure to sulfuric acid/ozone mixtures

Journal Article · Sun Jan 01 04:00:00 EST 1995 · Experimental Lung Research

DOI:https://doi.org/10.3109/01902149509031748· OSTI ID:114879

Chen, L C; Qu, Q; Amdur, M O ^[1]

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1. New York Univ. Medical Center, Tuxedo, NY (United States); and others

Recent studies have demonstrated that additive and synergistic effects on rabbit pulmonary macrophages (PMO) function can occur after combined exposures to acid aerosols and ozone. This study investigated intracellular pH (pH{sub i}) homeostasis and H{sup +} extrusion mechanisms of PMO from rabbits exposed to sulfuric acid, ozone, and their mixtures. Animals were exposed for 3 h to 125 {mu}g/m{sup 3} sulfuric acid, 0.1, 0.3, 0.6 ppm ozone, or combinations of acid with each concentration of ozone, and the Ph{sub i} was determined by a fluorescent dye ratioing technique. Exposure to 125 {mu}g/m{sup 3} acid reduced pH{sub i} and exposure to ozone resulted in a concentration-dependent reduction in pH{sub i}. Ozone generally tended to mitigate the effect of the acid aerosol on pH{sub i}. Other groups of rabbits were exposed to 50 {mu}g/m{sup 3} sulfuric acid, 0.6 ppm ozone, or their mixture, for 3 h, and PMO were again harvested. The pH{sub i} of PMO following exposure to each of the pollutant atmospheres was not different from control. However, H{sup +} extrusion with an imposed internal acid load was found to be significantly depressed following exposure to either sulfuric acid or ozone alone, while the mixture produced a significant interaction. 34 refs., 4 figs., 3 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 114879
• Journal Information: Experimental Lung Research, Journal Name: Experimental Lung Research Journal Issue: 1 Vol. 21; ISSN 0190-2148; ISSN EXLRDA
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL EFFECTS
INHALATION
LUNGS
MACROPHAGES
OZONE
PH VALUE
SULFURIC ACID