Increased accumulation of specific carotenoids in plastids through plant breeding or genetic engineering requires an understanding of the limitations that storage sites for these compounds may impose on that accumulation. Here, using Capsicum annuum L. fruit, we demonstrate directly the unique sub-organellar accumulation sites of specific carotenoids using live cell hyperspectral confocal Raman microscopy. Further, we show that chromoplasts from specific cultivars vary in shape and size, and these structural variations are associated with carotenoid compositional differences. Live-cell imaging utilizing laser scanning confocal (LSCM) and confocal Raman microscopy, as well as fixed tissue imaging by scanning and transmission electron microscopy (SEM and TEM), all demonstrated morphological differences with high concordance for the measurements across the multiple imaging modalities. These results reveal additional opportunities for genetic controls on fruit color and carotenoid-based phenotypes.
Kilcrease, James, et al. "Multiple microscopic approaches demonstrate linkage between chromoplast architecture and carotenoid composition in diverse <em><scp>C</scp>apsicum annuum</em> fruit." The Plant Journal, vol. 76, no. 6, Nov. 2013. https://doi.org/10.1111/tpj.12351
Kilcrease, James, Collins, Aaron M., Richins, Richard D., Timlin, Jerilyn A., & O'Connell, Mary A. (2013). Multiple microscopic approaches demonstrate linkage between chromoplast architecture and carotenoid composition in diverse <em><scp>C</scp>apsicum annuum</em> fruit. The Plant Journal, 76(6). https://doi.org/10.1111/tpj.12351
Kilcrease, James, Collins, Aaron M., Richins, Richard D., et al., "Multiple microscopic approaches demonstrate linkage between chromoplast architecture and carotenoid composition in diverse <em><scp>C</scp>apsicum annuum</em> fruit," The Plant Journal 76, no. 6 (2013), https://doi.org/10.1111/tpj.12351
@article{osti_1114129,
author = {Kilcrease, James and Collins, Aaron M. and Richins, Richard D. and Timlin, Jerilyn A. and O'Connell, Mary A.},
title = {Multiple microscopic approaches demonstrate linkage between chromoplast architecture and carotenoid composition in diverse <em><scp>C</scp>apsicum annuum</em> fruit},
annote = {Increased accumulation of specific carotenoids in plastids through plant breeding or genetic engineering requires an understanding of the limitations that storage sites for these compounds may impose on that accumulation. Here, using Capsicum annuum L. fruit, we demonstrate directly the unique sub-organellar accumulation sites of specific carotenoids using live cell hyperspectral confocal Raman microscopy. Further, we show that chromoplasts from specific cultivars vary in shape and size, and these structural variations are associated with carotenoid compositional differences. Live-cell imaging utilizing laser scanning confocal (LSCM) and confocal Raman microscopy, as well as fixed tissue imaging by scanning and transmission electron microscopy (SEM and TEM), all demonstrated morphological differences with high concordance for the measurements across the multiple imaging modalities. These results reveal additional opportunities for genetic controls on fruit color and carotenoid-based phenotypes.},
doi = {10.1111/tpj.12351},
url = {https://www.osti.gov/biblio/1114129},
journal = {The Plant Journal},
issn = {ISSN 0960-7412},
number = {6},
volume = {76},
place = {United States},
publisher = {Society for Experimental Biology},
year = {2013},
month = {11}}
Washington Univ., St. Louis, MO (United States); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Organization:
USDOE SC Office of Basic Energy Sciences (SC-22)
DOE Contract Number:
SC0001035
OSTI ID:
1114129
Report Number(s):
DE-SC0001035-98
Journal Information:
The Plant Journal, Journal Name: The Plant Journal Journal Issue: 6 Vol. 76; ISSN 0960-7412