Toxicology Studies on Lewisite and Sulfur Mustard Agents: Mutagenicity of Lewisite in the Salmonella Histidine Reversion Assay (Final Report)
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
The mutagenic potential of lewisite was evaluated in the standard plate incorporation method and by the preincubation modification of the Ames Salmonella/microsomal assay with tester strains TA97, TA98, TA100 and TA102. All strains were tested with activation (20 and 50 μl/plate) and without activation. The lewisite was screened initially for toxicity with TA98 over a range of concentrations from 0.01 to 250 μg of material per plate. However, concentrations selected for mutagenicity testing were adjusted to a range of 0.001 to 5 μg/plate because of the sensitivity of tester strain TA102, which exhibited cytotoxicity at 0.01 ug/plate. No mutagenic response was exhibited by any of the strains in either method used. All other tester strains showed evidence of cytoxicity (reduction in mutagen response or sparse background lawn) at 5.0 μg/plate or lower.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE; U.S. Army Medical Research and Development Command
- OSTI ID:
- 1086508
- Report Number(s):
- PNL--6872
- Country of Publication:
- United States
- Language:
- English
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