Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture

Patent · Tue Dec 25 00:00:00 EST 2012

OSTI ID:1082141

Beer, Neil R.; Hindson, Benjamin J.; Colson, Jr., Billy W.; Fitch, Joseph P.

A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-48
• Assignee: Lawrence Livermore National Security, LLC (Livermore, CA)
• Patent Number(s): 8,338,166
• Application Number: 11/650,363
• OSTI ID: 1082141
• Resource Relation: Patent File Date: 2007 Jan 04
• Country of Publication: United States
• Language: English