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Title: Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture

Abstract

A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.

Inventors:
; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1082141
Patent Number(s):
8,338,166
Application Number:
11/650,363
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Jan 04
Country of Publication:
United States
Language:
English

Citation Formats

Beer, Neil R., Hindson, Benjamin J., Colson, Jr., Billy W., and Fitch, Joseph P. Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture. United States: N. p., 2012. Web.
Beer, Neil R., Hindson, Benjamin J., Colson, Jr., Billy W., & Fitch, Joseph P. Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture. United States.
Beer, Neil R., Hindson, Benjamin J., Colson, Jr., Billy W., and Fitch, Joseph P. 2012. "Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture". United States. https://www.osti.gov/servlets/purl/1082141.
@article{osti_1082141,
title = {Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture},
author = {Beer, Neil R. and Hindson, Benjamin J. and Colson, Jr., Billy W. and Fitch, Joseph P.},
abstractNote = {A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.},
doi = {},
url = {https://www.osti.gov/biblio/1082141}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 25 00:00:00 EST 2012},
month = {Tue Dec 25 00:00:00 EST 2012}
}

Works referenced in this record:

Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume Droplets
journal, March 2005


BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions
journal, July 2006


Overview: methods and applications for droplet compartmentalization of biology
journal, July 2006


Amplification of complex gene libraries by emulsion PCR
journal, July 2006


APPLIED PHYSICS: Droplet Control for Microfluidics
journal, August 2005


Single-molecule PCR using water-in-oil emulsion
journal, April 2003