Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
Abstract
A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.
- Inventors:
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1082141
- Patent Number(s):
- 8,338,166
- Application Number:
- 11/650,363
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2007 Jan 04
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Beer, Neil R., Hindson, Benjamin J., Colson, Jr., Billy W., and Fitch, Joseph P. Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture. United States: N. p., 2012.
Web.
Beer, Neil R., Hindson, Benjamin J., Colson, Jr., Billy W., & Fitch, Joseph P. Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture. United States.
Beer, Neil R., Hindson, Benjamin J., Colson, Jr., Billy W., and Fitch, Joseph P. 2012.
"Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture". United States. https://www.osti.gov/servlets/purl/1082141.
@article{osti_1082141,
title = {Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture},
author = {Beer, Neil R. and Hindson, Benjamin J. and Colson, Jr., Billy W. and Fitch, Joseph P.},
abstractNote = {A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.},
doi = {},
url = {https://www.osti.gov/biblio/1082141},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 25 00:00:00 EST 2012},
month = {Tue Dec 25 00:00:00 EST 2012}
}
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