An Automated Platform for High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

Journal Article · Tue Oct 02 04:00:00 EDT 2012 · Analytical Chemistry, 84(19):8351-8356

DOI:https://doi.org/10.1021/ac301909a· OSTI ID:1053349

Lanekoff, Ingela T; Heath, Brandi S; Liyu, Andrey V; Thomas, Mathew; Carson, James P; Laskin, Julia

An automated platform has been developed for acquisition and visualization of mass spectrometry imaging (MSI) data using nanospray desorption electrospray ionization (nano-DESI). The new system enables robust operation of the nano-DESI imaging source over many hours. This is achieved by controlling the distance between the sample and the probe by mounting the sample holder onto an automated XYZ stage and defining the tilt of the sample plane. This approach is useful for imaging of relatively flat samples such as thin tissue sections. Custom software called MSI QuickView was developed for visualization of large data sets generated in imaging experiments. MSI QuickView enables fast visualization of the imaging data during data acquisition and detailed processing after the entire image is acquired. The performance of the system is demonstrated by imaging rat brain tissue sections. High resolution mass analysis combined with MS/MS experiments enabled identification of lipids and metabolites in the tissue section. In addition, high dynamic range and sensitivity of the technique allowed us to generate ion images of low-abundance isobaric lipids. High-spatial resolution image acquired over a small region of the tissue section revealed the spatial distribution of an abundant brain metabolite, creatine, in the white and gray matter that is consistent with the literature data obtained using magnetic resonance spectroscopy.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1053349
• Report Number(s): PNNL-SA-89054; 44679
• Journal Information: Analytical Chemistry, 84(19):8351-8356, Journal Name: Analytical Chemistry, 84(19):8351-8356 Journal Issue: 19 Vol. 84; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory
imaging mass spectrometry
nanospray desorption electrospray ionization (nano-DESI)
tissue
automated platform
MSI QuickView
data visualization