Shotgun Approach for Quantitative Imaging of Phospholipids Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

Journal Article · Tue Feb 04 04:00:00 EST 2014 · Analytical Chemistry, 86(3):1872-1880

DOI:https://doi.org/10.1021/ac403931r· OSTI ID:1129320

Lanekoff, Ingela T.; Thomas, Mathew; Laskin, Julia

Mass spectrometry imaging (MSI) has been extensively used for determining spatial distributions of molecules in biological samples, and there is increasing interest in using MSI for quantification. Nanospray desorption electrospray ionization, or nano-DESI, is an ambient MSI technique where a solvent is used for localized extraction of molecules followed by nanoelectrospray ionization. Doping the nano-DESI solvent with carefully selected standards enables online quantification during MSI experiments. In this proof-of-principle study, we demonstrate this quantification approach can be extended to provide shotgun-like quantification of phospholipids in thin brain tissue sections. Specifically, two phosphatidylcholine (PC) standards were added to the nano-DESI solvent for simultaneous imaging and quantification of 22 PC species observed in nano-DESI MSI. Furthermore, by combining the quantitative data obtained in the individual pixels, we demonstrate quantification of these PC species in seven different regions of a rat brain tissue section.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1129320
• Report Number(s): PNNL-SA-99849; 44679
• Journal Information: Analytical Chemistry, 86(3):1872-1880, Journal Name: Analytical Chemistry, 86(3):1872-1880
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory
imaging mass spectrometry
localization
nanospray desorption electrospray ionization (nano-DESI)
phosphatidylcholine
phospholipids
quantification
quantitative nano-DESI imaging
rat brain