Differential growth responses of soil bacterial taxa to carbon substrates of varying chemical recalcitrance
Journal Article
·
· Frontiers in Terrestrial Micobiology
Soils are immensely diverse microbial habitats with thousands of co-existing bacterial, archaeal, and fungal species. Across broad spatial scales, factors such as pH and soil moisture appear to determine the diversity and structure of soil bacterial communities. Within any one site however, bacterial taxon diversity is high and factors maintaining this diversity are poorly resolved. Candidate factors include organic substrate availability and chemical recalcitrance, and given that they appear to structure bacterial communities at the phylum level, we examine whether these factors might structure bacterial communities at finer levels of taxonomic resolution. Analyzing 16S rRNA gene composition of nucleotide analog-labeled DNA by PhyloChip microarrays, we compare relative growth rates on organic substrates of increasing chemical recalcitrance of >2,200 bacterial taxa across 43 divisions/phyla. Taxa that increase in relative abundance with labile organic substrates (i.e., glycine, sucrose) are numerous (>500), phylogenetically clustered, and occur predominantly in two phyla (Proteobacteria and Actinobacteria) including orders Actinomycetales, Enterobacteriales, Burkholderiales, Rhodocyclales, Alteromonadales, and Pseudomonadales. Taxa increasing in relative abundance with more chemically recalcitrant substrates (i.e., cellulose, lignin, or tannin-protein) are fewer (168) but more phylogenetically dispersed, occurring across eight phyla and including Clostridiales, Sphingomonadalaes, Desulfovibrionales. Just over 6% of detected taxa, including many Burkholderiales increase in relative abundance with both labile and chemically recalcitrant substrates. Estimates of median rRNA copy number per genome of responding taxa demonstrate that these patterns are broadly consistent with bacterial growth strategies. Taken together, these data suggest that changes in availability of intrinsically labile substrates may result in predictable shifts in soil bacterial composition.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- Earth Sciences Division
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 1048271
- Report Number(s):
- LBNL-4963E
- Journal Information:
- Frontiers in Terrestrial Micobiology, Journal Name: Frontiers in Terrestrial Micobiology Vol. 2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Novelty and uniqueness patterns of rare members of the soil biosphere
Soil organic matter availability and climate drive latitudinal patterns in bacterial diversity from tropical to cold temperate forests
Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients
Journal Article
·
Tue Sep 30 00:00:00 EDT 2008
· Applied and Environmental Microbiology, 74(17):5422-5428
·
OSTI ID:947902
Soil organic matter availability and climate drive latitudinal patterns in bacterial diversity from tropical to cold temperate forests
Journal Article
·
Sun Aug 06 20:00:00 EDT 2017
· Functional Ecology
·
OSTI ID:1567107
Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients
Journal Article
·
Wed Nov 30 19:00:00 EST 2011
· The ISME Journal
·
OSTI ID:1623755