Contrasting colonization and plant growth promoting capacity between wild type and gfp-derative of the endophyte Pseudomonas putida W619 in hybrid poplar

Journal Article · Thu Jun 09 04:00:00 EDT 2011 · Plant and Soil

OSTI ID:1044000

Weyens, N; Lelie D, van der; Boulet, J; Adriaensen, D; Timmermans, J -P; Prinsen, E; Van Oevelen, S; D"Haen, J; Smeets, K; Taghavi, S; Vangronsveld, J

This study aims to investigate the colonization of poplar by the endophyte Pseudomonas putida W619 and its capacity to promote plant growth. Poplar cuttings were inoculated with P. putida W619 (wild-type or gfp-labelled). The colonization of both strains was investigated and morphological, physiological and biochemical parameters were analyzed to evaluate plant growth promotion. Inoculation with P. putida W619 (wild-type) resulted in remarkable growth promotion, decreased activities of antioxidative defence related enzymes, and reduced stomatal resistance, all indicative of improved plant health and growth in comparison with the non-inoculated cuttings. In contrast, inoculation with gfp-labelled P. putida W619 did not promote growth; it even had a negative effect on plant health and growth. Furthermore, compared to the wildtype strain, colonization by the gfp-labelled P. putida W619::gfp1 was much lower; it only colonized the rhizosphere and root cortex while the wild-type strain also colonized the root xylem vessels. Despite the strong plant growth promoting capacity of P. putida W619 (wild-type), after gfp labelling its growth promoting characteristics disappeared and its colonization capacity was strongly influenced; for these reasons gfp labelling should be applied with sufficient caution.

• Research Organization: BROOKHAVEN NATIONAL LABORATORY (BNL)
• Sponsoring Organization: USDOE SC OFFICE OF SCIENCE (SC)
• DOE Contract Number: AC02-98CH10886
• OSTI ID: 1044000
• Report Number(s): BNL--90171-2012-JA; KP1102010
• Journal Information: Plant and Soil, Journal Name: Plant and Soil Journal Issue: 1-2 Vol. 356; ISSN PLSOA2; ISSN 0032-079X
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
54 ENVIRONMENTAL SCIENCES
BIOMASS
CAPACITY
ENZYMES
INOCULATION
PLANT GROWTH
POPLARS
PRODUCTION
PSEUDOMONAS
STRAINS