Potential of the TCE-degrading endophyte Pseudomonas putida W619-TCE to improve plant growth and reduce TCE phytotoxicity and evapotranspiration in poplar cuttings

Journal Article · Wed Sep 01 04:00:00 EDT 2010 · Environmental Pollution

DOI:https://doi.org/10.1016/j.envpol.2010.06.004· OSTI ID:1014317

Weyens, N; van der Lelie, D; Truyens, S; Dupae, J; Newman, L; Taghavi, S; Carleer, R; Vangronsveld, J

The TCE-degrading poplar endophyte Pseudomonas putida W619-TCE was inoculated in poplar cuttings, exposed to 0, 200 and 400 mg l{sup -1} TCE, that were grown in two different experimental setups. During a short-term experiment, plants were grown hydroponically in half strength Hoagland nutrient solution and exposed to TCE for 3 days. Inoculation with P. putida W619-TCE promoted plant growth, reduced TCE phytotoxicity and reduced the amount of TCE present in the leaves. During a mid-term experiment, plants were grown in potting soil and exposed to TCE for 3 weeks. Here, inoculation with P. putida W619-TCE had a less pronounced positive effect on plant growth and TCE phytotoxicity, but resulted in strongly reduced amounts of TCE in leaves and roots of plants exposed to 400 mg l{sup -1} TCE, accompanied by a lowered evapotranspiration of TCE. Dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA), which are known intermediates of TCE degradation, were not detected. The endophyte P. putida W619-TCE degrades TCE during its transport through the xylem, leading to reduced TCE concentrations in poplar, and decreased TCE evapotranspiration.

• Research Organization: BROOKHAVEN NATIONAL LABORATORY (BNL)
• Sponsoring Organization: LABORATORY-DIRECTED RESEARCH AND DEVELOPMENT
• DOE Contract Number: AC02-98CH10886
• OSTI ID: 1014317
• Report Number(s): BNL--90701-2010-JA; YN0100000
• Journal Information: Environmental Pollution, Journal Name: Environmental Pollution Journal Issue: 9 Vol. 158; ISSN 0269-7491; ISSN ENPOEK
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
INOCULATION
NUTRIENTS
PLANT GROWTH
POPLARS
POTTING
PSEUDOMONAS
Pseudomonas putida W619-TCE
SOILS
TRANSPORT
endophyte
phytoremediation
poplar
trichloroethylene