Antitumor Activity of Peptide Amphiphile Nanofiber-Encapsulated Camptothecin

Journal Article · Mon Apr 02 04:00:00 EDT 2012 · ACS Nano

DOI:https://doi.org/10.1021/nn203343z· OSTI ID:1034183

Soukasene, Stephen; Toft, Daniel J; Moyer, Tyson J; Lu, Hsuming; Lee, Hyung-Kun; Standley, Stephany M; Cryns, Vincent L; Stupp, Samuel I ^[1]

---

1. NWU

Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.

• Research Organization: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
• Sponsoring Organization: NCI
• OSTI ID: 1034183
• Journal Information: ACS Nano, Journal Name: ACS Nano Journal Issue: (11) ; 11, 2011 Vol. 5
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

60 APPLIED LIFE SCIENCES
CHEMOTHERAPY
DISEASES
ENCAPSULATION
EVAPORATION
IN VITRO
MAMMARY GLANDS
NEOPLASMS
PEPTIDES
SCATTERING
SOLUBILITY
SOLVENTS
THERAPY
TRANSMISSION ELECTRON MICROSCOPY