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Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Patent ·
OSTI ID:1029285
 [1];  [2];  [3];  [4]
  1. San Francisco, CA
  2. Brookline, MA
  3. Cambridge, MA
  4. La Jolla, CA
+ Show Author Affiliations

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.

Research Organization:
The Scripps Research Institute (La Jolla, CA)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-00ER45812
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,030,028
Application Number:
12/590,742
OSTI ID:
1029285
Country of Publication:
United States
Language:
English

References (34)

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  • Journal of the American Chemical Society, Vol. 125, Issue 4, p. 935-939 https://doi.org/10.1021/ja0284153
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