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Title: Pressure cryocooling protein crystals

Abstract

Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.

Inventors:
 [1];  [1]
  1. Ithaca, NY
Publication Date:
Research Org.:
Cornell University
Sponsoring Org.:
USDOE
OSTI Identifier:
1029280
Patent Number(s):
8,030,449
Application Number:
11/700,237
Assignee:
Cornell Research Foundation, Inc. (Ithaca, NY) CHO
DOE Contract Number:  
FG02-97ER62443
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Kim, Chae Un, and Gruner, Sol M. Pressure cryocooling protein crystals. United States: N. p., 2011. Web.
Kim, Chae Un, & Gruner, Sol M. Pressure cryocooling protein crystals. United States.
Kim, Chae Un, and Gruner, Sol M. Tue . "Pressure cryocooling protein crystals". United States. https://www.osti.gov/servlets/purl/1029280.
@article{osti_1029280,
title = {Pressure cryocooling protein crystals},
author = {Kim, Chae Un and Gruner, Sol M},
abstractNote = {Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {10}
}

Patent:

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