Pressure cryocooling protein crystals

Kim, Chae Un; Gruner, Sol M

Pressure cryocooling protein crystals

Patent · Tue Oct 04 00:00:00 EDT 2011

OSTI ID:1029280

Kim, Chae Un ^[1]; Gruner, Sol M ^[1]

Ithaca, NY

Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.

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Research Organization:: Cornell University

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-97ER62443

Assignee:: Cornell Research Foundation, Inc. (Ithaca, NY)

Patent Number(s):: 8,030,449

Application Number:: 11/700,237

OSTI ID:: 1029280

Country of Publication:: United States

Language:: English

References (14)

Crystallizing a Protein Drenth, Jan Principles of Protein X-ray Crystallography https://doi.org/10.1007/978-1-4757-2335-9_1	book	January 1994
Use of Noble Gases Xenon and Krypton as Heavy Atoms in Protein Structure Determination Schiltz, Marc; Fourme, Roger; Prangé, Thierry Methods in Enzymology, Vol. 374, p. 83-119 https://doi.org/10.1016/S0076-6879(03)74004-X	book	January 2003
Models of protein crystal growth Kierzek, Andrzej M.; Zielenkiewicz, Piotr Biophysical Chemistry, Vol. 91, Issue 1 https://doi.org/10.1016/S0301-4622(01)00157-0	journal	June 2001
Probing Substates in Sperm Whale Myoglobin Using High-Pressure Crystallography Urayama, Paul; Phillips, George N.; Gruner, Sol M. Structure, Vol. 10, Issue 1 https://doi.org/10.1016/S0969-2126(01)00699-2	journal	January 2002
The RCK Domain of the KtrAB K+ Transporter: Multiple Conformations of an Octameric Ring Albright, Ronald A.; Ibar, José-Luís Vazquez; Kim, Chae Un Cell, Vol. 126, Issue 6 https://doi.org/10.1016/j.cell.2006.08.028	journal	September 2006
Protein recognition of macrocycles: Binding of anti-HIV metallocyclams to lysozyme Hunter, T. M.; McNae, I. W.; Liang, X. Proceedings of the National Academy of Sciences, Vol. 102, Issue 7 https://doi.org/10.1073/pnas.0407595102	journal	February 2005
Freezing of myoglobin crystals at high pressure Thomanek, U. F.; Parak, F.; Mössbauer, R. L. Acta Crystallographica Section A, Vol. 29, Issue 3 https://doi.org/10.1107/S0567739473000677	journal	May 1973
MAD phasing with krypton Cohen, Aina; Ellis, Paul; Kresge, Nicole Acta Crystallographica Section D Biological Crystallography, Vol. 57, Issue 2 https://doi.org/10.1107/S0907444900014670	journal	February 2001
High-pressure cooling of protein crystals without cryoprotectants Kim, Chae Un; Kapfer, Raphael; Gruner, Sol M. Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 7 https://doi.org/10.1107/S090744490500836X	journal	June 2005
Crystal growth in X-ray-transparent plastic tubing: an alternative for high-throughput applications Kalinin, Yevgeniy; Thorne, Robert Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 11 https://doi.org/10.1107/S0907444905028003	journal	October 2005
Solution of protein crystallographic structures by high-pressure cryocooling and noble-gas phasing Kim, Chae Un; Hao, Quan; Gruner, Sol M. Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 7 https://doi.org/10.1107/S0907444906014727	journal	June 2006
Crystallogenesis of biological macromolecules: facts and perspectives Giegé, R.; Lorber, B.; Théobald-Dietrich, A. Acta Crystallographica Section D Biological Crystallography, Vol. 50, Issue 4 https://doi.org/10.1107/S0907444994001344	journal	July 1994
New Strategies for Protein Crystal Growth Wiencek, J. M. Annual Review of Biomedical Engineering, Vol. 1, Issue 1 https://doi.org/10.1146/annurev.bioeng.1.1.505	journal	August 1999
Simple Helium Enclosure for Increasing Diffraction-Line Detectability with a G.E. XRD-5 Diffractometer Johnson, Harold A. Applied Spectroscopy, Vol. 22, Issue 3 https://doi.org/10.1366/000370268774383525	journal	May 1968

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